在Yarrowia lipolytica中的卢丁生物合成
Zhilei Qin1,2, Mengsu Liu1,2, Xuefeng Ren1,2
1Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
Journal of agricultural and food chemistry
|February 27, 2024
概括
在Yarrowia lipolytica中,通过优化前体路径和酶局部化,增强了微生物对黄蛋白的合成. 这实现了675.40毫克/升的黄蛋白标位记录,证明了可持续的生产方法.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 氨酸是一种有价值的胡卜素,在料,化品,食品和药品中具有多种应用.
- 微生物蛋白合成为植物提取提供了可持续的替代品,但在前体供应和酶效率方面面临挑战.
- 雅罗维亚 (Yarrowia lipolytica) 是微生物胡卜素生产的一个有前途的宿主.
研究的目的:
- 为了增强Yarrowia lipolytica中的微生物蛋白的合成.
- 为了克服前体可用性和低酶活性在蛋白生物合成中的局限性.
- 为了实现潜在的工业应用,实现高蛋白标位.
主要方法:
- 增强甲基酸盐 (MVA) 途径以增加前体供应.
- 在Yarrowia lipolytica中,关键酶的亚细胞局部化,包括α-胡卜素合成酶OuluLCY和基酶.
- 使用截断的N端运输来优化基酶活性.
- 在5升生物反应器中进行扩大规模的研究,并补充抗氧化剂.
主要成果:
- 阿尔法胡卜素标位增加了10.50倍,达到38.50 mg/L.
- 开发出最终的L9菌株,达到75.25mg/L的黄蛋白.
- 在5L生物反应器中,创纪录的黄蛋白标位为675.40 mg/L (6.13 mg/g DCW).
- 在Yarrowia lipolytica中成功地进行了蛋白的新生合成.
结论:
- 这项研究成功地在Yarrowia lipolytica中成功建立了de novo黄蛋白合成.
- 优化代谢途径和酶局部化显著提高了黄蛋白的生产.
- 实现的黄蛋白标位代表了微生物生产的突破,提供了一种可持续和高效的方法.
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