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闪光-达尔加诺可访问性控制了与亚第宁核糖核糖交换机的核糖体结合

Julius Blechar¹, Vanessa de Jesus¹, Boris Fürtig¹

¹Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Straße 7, 60438 Frankfurt am Main, Germany.

ACS chemical biology

|February 27, 2024

概括

腺素感应的核糖开关通过改变RNA结构来控制基因表达. 这项研究表明,腺结合增加了核糖体结合部位的可访问性,核糖体蛋白S1进一步增强了这种效应,以有效调节基因.

科学领域:

分子生物学分子生物学
在RNA生物学,RNA生物学.
基因规则基因规则

背景情况:

翻译性核糖突变调节基因表达通过调节核糖体结合部位 (RBS) 可访问性通过连接体诱导的构造变化.
了解这些动态结构变化对于破译基因调节机制至关重要.

研究的目的:

为了研究*Vibrio vulnificus*中的腺素感应核糖开关.
为了量化无联体 (apo) 和联体 (adenine-bound) 状态之间的RBS可访问性的变化.
为了阐明核糖体蛋白S1在核糖开关调节中的作用.

主要方法:

采用的单分子聚合技术:单分子RNA短暂结构动态分析 (SiM-KARTS) 和单分子核糖体结合动态分析 (SiM-KARB).
利用微尺度热泳 (MST) 来分析连接体结合和构造变化.
确定核糖体结合和核糖体开关状态转换的动力速率常数.

主要成果:

无论是SiM-KARTS还是SiM-KARB,都能准确地报告RBS在腺素感应核糖开关中的可访问性.
氨酸结合显著增加了RBS的可访问性,将 рибо开关转换为"启动"状态.
核糖体蛋白S1促进RNA二级结构的解,增强核糖体在apo状态中的结合.

结论:

氨酸与核糖开关的结合增加了RBS的可访问性,促进了翻译启动.
核糖体蛋白S1在启动核糖体开关以进行核糖体结合中起着关键作用.
与 рибо开关状态转换相比,较快的核糖体结合动力学确保了高效的监管决策.

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