通过N端酸化对阿拉比多普西6-酸酸脱酶异型的双重向控制的证据
Lennart Nico Doering1, Niklas Gerling1, Loreen Linnenbrügger1
1University of Münster, Department of Biology, Institute of Plant Biology and Biotechnology, Molecular Physiology of Plants, Schlossplatz 7, D-48149 Münster, Germany.
Journal of experimental botany
|February 27, 2024
概括
氧化酸通路酶PGD2在受精过程中准过氧体,由酸化调节. 这种定位开关对于植物繁殖至关重要,并与种子植物一起进化.
科学领域:
- 植物生物学 植物生物学
- 分子和细胞生物学分子和细胞生物学
- 生物化学 生物化学
背景情况:
- 氧化酸路径 (OPPP) 对于植物塑性质中NADPH的产生至关重要.
- 之前的研究探讨了OPPP酶的过渡性过氧体局部化,但第三种酶的机制仍然不清楚.
- 了解酶亚细胞局部化是代谢调节的关键.
研究的目的:
- 阐明阿拉比多普西的6 - 糖酸脱酶2 (PGD2) 的过氧体向机制.
- 为了研究PGD2在受精过程中的过氧体中的作用.
- 探索植物PGD同类物中超氧体向信号的进化意义.
主要方法:
- 在Arabidopsis中进行遗传分析.
- 酵母两杂交 (Y2H) 和双分子光补充 (BiFC) 试验以确认蛋白质相互作用.
- 构建和分析PGD-reporter合并,包括介质报告器合并.
- 局部定向的突变发生,以产生相仿性变化.
主要成果:
- 在受精期间,阿拉比多普西斯PGD2在过氧体中不可或缺.
- 在PGD2中Thr6的相仿变化诱导了切换到过氧体向.
- 在PGD2的N端的酸化干扰了细胞质二元化,暴露了C端的过氧体向信号 (PTS1).
- 在PGD同类物体中发现了PTS1动图,它们首先出现在.
结论:
- N端酸化通过调节二元化和暴露一个PTS1.1来调节PGD2亚细胞局部化.
- 在Arabidopsis中,PGD2的过氧体向对于受精至关重要.
- 在PGD同类物种中PTS1图案的演变与种子植物的出现及其繁殖策略相关.
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