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LncRNA Gm15232通过miR-192-3p/GCR通路促进老化小鼠的脂质发生

Yaqi Hu^1,2, Yifan Zhang^1,2, Shuwen Wang^1,2

¹Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, China.

The journals of gerontology. Series A, Biological sciences and medical sciences

|February 27, 2024

概括

长链非编码RNAs (lncRNAs) 对代谢疾病产生影响. 这项研究发现,lncRNA Gm15232通过抑制miR-192-3p来促进老年小鼠的脂肪合成,这为与年龄相关的脂质代谢障碍提供了潜在的治疗标.

科学领域:

分子生物学分子生物学
老年学是一门学科.
代谢研究研究代谢研究

背景情况:

长链非编码RNAs (lncRNAs) 与代谢疾病有关.
lncRNAs在与年龄相关的脂质合成失调中的作用尚不清楚.
衰老显著扰乱了正常的脂质代谢.

研究的目的:

研究 lncRNAs 在老年小鼠脂质代谢中的作用.
为了确定特定的 lncRNAs 影响异常脂质合成在衰老过程中.
阐明老年人中 lncRNA介导的脂质生成背后的分子机制.

主要方法:

在成年和老化小鼠的脂肪组织中对lncRNA表达的比较分析.
调查lncRNA Gm15232作为竞争的内源RNA (ceRNA) 的功能.
对Gm15232对miR-192-3p表达的抑制作用的评估.
评估miR-192-3p对葡萄糖皮质体受体基因表达和随后的脂质生成的影响.

主要成果:

在老化小鼠的 epididymal 白脂肪组织中,LncRNA Gm15232 表达显著上调.
上调的Gm15232抑制了miR-192-3p的表达,作为一个ceRNA.
降低miR-192-3p的调节导致葡萄糖皮质体受体基因表达的增加.
这一途径最终刺激了脂肪合成,表明Gm15232促进了脂质生成.

结论:

LncRNA Gm15232在促进与年龄相关的脂肪合成方面发挥着至关重要的作用.
Gm15232/miR-192-3p/葡萄皮质体受体轴是与年龄相关的脂质发生的一个关键机制.
向lncRNA Gm15232为与年龄相关的代谢异常提供了潜在的治疗策略.

关键词:

在Gm1523222中使用.老化的老化衰老的老化葡萄糖皮质类受体受体脂质生成是指脂肪的产生.在 miR-192-3pp 中.

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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...