在高温下对Naematelia aurantialba的适应性实验室进化,以高效地生产外聚糖
Tao Sun1, Hao Jiang1, Xiaoyi Xu1
1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
International journal of biological macromolecules
|February 27, 2024
概括
适应性实验室进化增强了Naematelia aurantialba对于多糖生产的耐热性. 适应的菌株显示出增加的外聚糖产量和在更高的温度下优异的冷保护.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- 液体发酵为中多糖生产提供了潜在的潜力.
- 低温的限制限制了当前发酵效率.
- 提高耐热性对于工业可扩展性至关重要.
研究的目的:
- 使用适应性实验室进化 (ALE) 来提高Naematelia aurantialba的耐热性.
- 在高温下增加N. aurantialba中的外聚糖 (EPS) 生产.
- 为了描述适应菌株产生的EPS.
主要方法:
- 适应性实验室进化 (ALE) 在30°C的75个周期内.
- 在7.5L的发酵器中发酵.
- 对外聚糖化合物组成,分子量和冷保护的分析.
- 评估活性氧物种 (ROS) 水平和抗氧化酶活性.
- 转录基因和脂肪酸分析.
主要成果:
- 该ALE菌株的耐温度比野生型菌株高5°C.
- 与25°C的野生类型相比,EPS产量在30°C时增加了17%.
- 来自ALE的EPS具有更大的分子量和增强的冷保护.
- 适应菌株显示ROS减少和抗氧化酶活性增加.
- 代谢分析显示了碳水化合物,氨基酸和脂质代谢的重新配置.
结论:
- ALE对于开发耐热N.aurantialba菌株的高温发酵是有效的.
- 适应的菌株提供了更好的EPS生产和质量.
- 这种方法减少了多糖制造中的能源消耗和成本.
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