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通过与胆管细胞共同培养来调节肝细胞紧密结节,可实现非破坏性的胆汁恢复

Fumiya Tokito¹, Mikito Kiyofuji¹, Hyunjin Choi¹

¹Department of Chemical System Engineering, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

Journal of bioscience and bioengineering

|February 27, 2024

概括

这项研究引入了一种新的非破坏性方法,用于分析胆汁中的药物代谢物,使用共同培养的肝脏和胆道细胞. 这种技术增强了胆汁回收,以改善体外药物处置研究.

科学领域:

肝胆排放和药物代谢研究.

背景情况:

精确估计胆道药物清除值对于了解药物处置和相互作用至关重要.
目前使用三明治培养肝细胞的体外方法需要细胞破坏,限制了长期研究.

研究的目的:

开发一种非破坏性体外方法来分析胆道药物分泌.
研究共同培养肝脏 (HepG2-NIAS) 和胆管癌 (TFK-1) 细胞的可行性,以改善胆汁分析.

主要方法:

共同培养HepG2-NIAS和TFK-1细胞在一个原体维特里格尔膜上.
评估光素标记胆酸 (CLF) 和右的透性.
测量横体电阻 (TEER),以评估紧接口的完整性.
以非破坏性的方式从胆管中量化CLF恢复.

主要成果:

与TFK-1细胞共同培养,在HepG2-NIAS层中增加了1.2倍的CLF透性,这表明紧紧的结节松动了.
这种共同培养方法提高了CLF从胆管中恢复的1.4倍,而不会破坏紧密的结节.
TEER值下降,乳糖透性增加,证实了对细胞泄漏.

结论:

同培养HepG2-NIAS和TFK-1细胞提供了一种可行的非破坏性胆汁恢复方法.
这种方法为估计胆汁代谢物和推进体外药物分析提供了有价值的工具.
该方法有可能用于长期和重复剂量药物效应评估.

关键词:

胆汁收集胆汁收集基于细胞的测定方法.胆基细胞 (Cholangiocytes) 是一种细胞.培养培养培养这是一种HepG2-NIASAS.肝细胞肝细胞肝细胞不具有破坏性的非破坏性.在三明治培养肝细胞中培养的肝细胞.在TFK-1中使用.

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