通过接触式流深过产生基于复合囊泡性口腔炎病毒的载体
Sven Göbel1, Lars Pelz1, Cristina A T Silva2
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr. 1, 39106, Magdeburg, Germany.
Applied microbiology and biotechnology
|February 27, 2024
概括
一种新的触流深度过 (TFDF) perfusion 系统显著提高了病毒载体的产生. 这种封闭的系统可以持续收集和澄清病毒,实现更高的产量和加强疫苗和病毒治疗药物的生产.
科学领域:
- 生物技术和生物加工
- 病毒学和病毒载体生产
- 细胞培养和生物反应器技术
背景情况:
- 基于细胞培养的生产基于载体的疫苗和病毒治疗药物正在变得越来越重要.
- 现有的方法需要优化,以实现高效和持续的病毒收集和澄清.
- 基于复合囊泡性口腔炎病毒 (rVSV) 的载体对治疗应用具有前景.
研究的目的:
- 评估一个集成的封闭一次性输液系统,用于持续生产病毒载体.
- 评估触流深度过 (TFDF) 对于细胞保留,病毒收集和澄清的有效性.
- 为了比较TFDF perfusion系统的性能与优化的批量工艺.
主要方法:
- 在悬浮BHK-21和HEK293-SF细胞中使用两种基于rVSV的结构进行概念验证研究.
- 实施修改的交替接触流 (mATF) 装置和接触流深度过 (TFDF) 系统,用于细胞保留.
- 灌种植,持续收获和澄清,然后进行传染性病毒标位量化.
主要成果:
- 该TFDF系统实现了高可活细胞度 (VCC),高达20.6×10^6细胞/毫升.
- 通过使用TFDF系统,成功地证明了持续的载体采集和澄清.
- 与批量工艺相比,TFDF输液系统的传染性病毒标位高达11倍,时空产量增加了460%.
结论:
- TFDF perfusion 系统对于强化病毒载体生产是有效的,使得持续的收集和澄清成为可能.
- 这种综合系统在生产疫苗和病毒治疗药物的传统批量方法上提供了显著的优势.
- 该TFDF perfusion系统显示了建立强化和高效的病毒载体制造工艺的巨大潜力.
相关概念视频
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