一个C. crossoptilon的高质量基因组组合和相关的功能和遗传数据资源
Siwen Wu1, Kun Wang2,3, Tengfei Dou2
1Department of Bioinformatics and Genomics, College of Computing and Informatics, the University of North Carolina at Charlotte, Charlotte, NC, 28223, USA.
Scientific data
|February 27, 2024
概括
我们对白耳 (WT) 的基因组进行了测序,以了解危羊物种的高海拔适应性和遗传多样性. 这项研究为保护工作提供了关键的基因组资源.
科学领域:
- 基因组学就是基因组学.
- 进化生物学 进化生物学
- 保护科学 保护科学
背景情况:
- 四种十字羊在中国的不同高度生活.
- 这些鱼物种面临着不同的灭绝风险.
- 了解遗传适应对于它们的生存至关重要.
研究的目的:
- 为白耳 (Crossoptilon crossoptilon) 组装一个高质量的基因组.
- 研究该物种适应高海拔的遗传基础.
- 为保护临灭绝的羊提供基因组资源.
主要方法:
- 使用Illumina短读,PacBio长读和Hi-C技术进行基因组组.
- 来自白耳个体的20个主要组织的转录基因组测序.
- 10只白耳和1只蓝耳 (Crossoptilon auritum) 个体的全基因组重新测序.
主要成果:
- 一个高质量的基因组组 (~1.02Gb) 的白耳被生成与一个contigN50的19.63Mb.
- 为了进一步的遗传分析,获得了转录组和人口测序数据.
- 这项研究为Crossoptilon属提供了宝贵的基因组资源.
结论:
- 组装的基因组和相关数据对于研究鸟生物学和进化非常有价值.
- 这些资源将有助于了解危羊种群的高海拔适应性和遗传变化.
- 这些发现有助于制定有效的Crossoptilon物种保护战略.
更多相关视频
00:06In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
Published on: August 20, 2019
13.6K
09:06High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
Published on: October 5, 2018
10.3K
相关概念视频
Genome Annotation and Assembly
18.8K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.8K
Evolutionary Relationships through Genome Comparisons
5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.7K
RNA-seq
10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K
Multi-species Conserved Sequences
3.9K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
3.9K
Genomics
36.3K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.3K
Next-generation Sequencing
88.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.8K
