核心时钟基因调整了木的生长停止时间,使其日夜交换
Daniel Alique1, Arturo Redondo López1, Nahuel González Schain1,2
1Centro de Biotecnología y Genómica de Plantas (CBGP, UPM-INIA) Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA, CSIC), Campus de Montegancedo, Pozuelo de Alarcón, 28223, Madrid, Spain.
Nature communications
|February 27, 2024
概括
树利用日长来通过FT2基因控制季节性生长. 核心时钟基因,包括GIGANTEA (GI),CAB表达的时间 (TOC1) 和LATE ELONGATED HYPOCOTYL (LHY2),调节FT2表达,从而实现精确的季节性时间.
科学领域:
- 植物生物学 植物生物学
- 循环节律是指循环节律的节奏.
- 分子遗传学 分子遗传学
背景情况:
- 树利用光周期来确定季节性时间,影响植物表观和年度生长周期.
- 开花地点T 2 (FT2) 基因表达对于发芽顶部的发展至关重要,并由日长线索调节.
- 了解控制FT2转录及其在阿拉比多普西斯开花时的保存的分子机制是有限的.
研究的目的:
- 为了研究控制树FT2转录的分子因素.
- 阐明核心时钟基因在调解FT2表达的光周期控制中的作用.
- 建立一个基于数据的模型,将时钟基因与每日FT2水平和生长停止联系起来.
主要方法:
- 在对光周期的反应中对FT2表达的定量分析.
- 开发一个最小的数据驱动模型,将核心时钟基因 (GI,TOC1,LHY2) 与FT2水平连接起来.
- 使用CRISPR/Cas9基因编辑来创建功能丧失线,用于验证基因角色.
主要成果:
- FT2表达量化介导着树的生长停止反应.
- GIGANTEA (GI) 作为FT2激活窗口的关键诱导器.
- 电缆表达的时间 (TOC1) 和延迟延长的海波 (LHY2) 抑制FT2表达,TOC1被确定为一个关键的抑制器.
结论:
- 一个涉及核心时钟基因的昼夜介导机制调节了树的FT2表达.
- FT2水平作为一个准确的日长计,使树能够根据日长的缩短来调整生长停止.
- 这项研究确定TOC1是生长的光周期控制中的关键抑制剂.
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