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快速,视觉,基于标签的生物传感器平台,用于在临床应用中识别型肝炎病毒

Yuanfang Shi^1,2, Qingxue Zhou³, Shilei Dong⁴

¹The Second Clinical Medical College, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, 550003, People's Republic of China.

BMC microbiology

|February 27, 2024

概括

一种新的C型肝炎病毒 (HCV) 诊断方法,反转录循环介导的同热放大,与基于金纳米粒子的侧流生物传感器 (RT-LAMP-AuNPs-LFB) 集成,提供快速,灵敏和视觉识别. 这种具有成本效益的测试显示了对临床诊断的潜力,特别是在资源有限的环境中.

科学领域:

分子诊断学分子诊断学
肝炎C病毒 (HCV) 检测检测
异热放大技术是一种异热放大技术.

背景情况:

型肝炎病毒 (HCV) 感染仍然是一个重大的全球健康问题.
准确和快速的诊断工具对于有效的管理和控制至关重要.
现有的诊断方法可能需要专门的设备或漫长的程序.

研究的目的:

开发和评估一种用于HCV识别的新型分子诊断方法.
创建一个快速,灵敏,具体,简单,和视觉测定HCV检测.
评估开发的试验对治疗场所应用的潜力.

主要方法:

设计了新的反转录循环介导的异热放大 (RT-LAMP) 起始器,针对HCV5'未翻译区域 (5'UTR).
集成RT-LAMP与基于金纳米粒子的侧流生物传感器 (AuNPs-LFB) 进行视觉检测.
优化了测定条件,并使用临床样本评估了灵敏度,特异性和选择性.

主要成果:

成功设计了针对流行HCV基因型的特定RT-LAMP原料.
整个测定,包括样品准备,RT-LAMP和视觉检测,在40分钟内完成.
每次测试达到20个副本的检测限制,具有高特异性和抗干扰能力.

结论:

开发的HCV-RT-LAMP-AuNPs-LFB测定是一种敏感,特定,快速,视觉和节省成本的HCV识别方法.
这种诊断方法显示出对HCV的临床诊断有很大的潜力.
这种测试对资源有限的地区尤其有希望,因为这些地区需要可访问的诊断解决方案.

关键词:

生物传感器是一种生物传感器.肝炎C病毒感染者检测的检测极限护理点平台的平台.反转录环介导的同热放大反转录环介导的同热放大.

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