皮肤第三者烟雾暴露诱导了通过氧化损伤和MMP-1表达的人类角质细胞的表皮改变
Rengin Reis1, Kubra Kolci1,2, Eda Nil Yedikardes1
1Department of Toxicology, Faculty of Pharmacy, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey.
Experimental dermatology
|February 28, 2024
概括
第三手烟 (THS) 对皮肤细胞有毒,导致损伤和延迟愈合. 这项研究表明,THS暴露会损害人类的角质细胞,强调其作为环境污染物的风险.
科学领域:
- 环境健康 环境健康
- 皮肤病学 皮肤病学
- 毒理学 毒理学 毒理学
背景情况:
- 第三手烟 (THS) 包含表面的残留香烟烟雾,造成皮肤暴露的风险.
- 室内环境中的THS积累是一个日益严重的公共卫生问题.
- 了解THS皮肤毒性对于公众健康意识至关重要.
研究的目的:
- 为了研究皮肤第三手烟 (THS) 暴露对HaCaT人类角质细胞的毒性影响.
- 评估THS对细胞功能的影响,包括细胞毒性,氧化应激和伤口愈合.
- 评估硫福拉 (SFN) 对THS诱导的皮肤损伤的潜在保护作用.
主要方法:
- 从受污染的布中提取THS并应用于HaCaT细胞.
- 细胞毒性测试 (MTT,NRU) 用于测量细胞活力.
- 评估细胞内谷氨 (GSH),总超氧化解突变酶 (SOD) 活性,MMP-1和IL-6水平.
- 划伤试验用于评估角质细胞的伤口愈合能力.
- 作为THS毒性防护剂使用的SFN.
主要成果:
- 通过线粒体功能障碍,THS在HaCaT细胞中诱导了剂量依赖的细胞毒性.
- 在SFN治疗前,THS诱导的细胞毒性显著改善.
- 暴露于THS降低了细胞内GSH和T-SOD活性,表明氧化应激.
- 观察到MMP-1表达和IL-6水平的升高,以及伤口愈合的延迟.
- THS证明了对皮肤细胞健康和功能的剂量依赖的负面影响.
结论:
- 通过皮肤接触第三者烟雾 (THS) 对人类角质细胞有细胞毒性.
- THS破坏细胞功能,增加氧化应激,并影响伤口愈合.
- 这些发现强调了THS作为环境皮肤污染物的重要性.
- 对THS皮肤暴露的进一步研究是有必要的,以了解皮肤疾病.
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