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Updated: May 8, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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可编程DNA条形码编码的指数放大反应用于miRNAs的多重检测

Yuqian Tan1, Li Zhang1, Shixiong Deng1

  • 1Department of Forensic Medicine, Chongqing Medical University, Chongqing 400016, China. dengshixiong@cqmu.edu.cn.

Analytical methods : advancing methods and applications
|February 28, 2024
PubMed
概括

这项研究提出了一种新的方法,用于同时检测多个microRNAs (miRNAs),使用指数异热放大 (E-EXPAR). 这种敏感的平台为早期疾病诊断和通过核酸检测监测提供了一个有前途的工具.

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相关概念视频

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...