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在1650 nm的相对照反射共聚焦脑成像时进行相对照反射

Patrick Delafontaine Martel^1,2, Cong Zhang^1,2, Andreas A Linninger³

¹Polytechnique Montreal, Department of Electrical Engineering, Montreal, Québec, Canada.

Journal of biomedical optics

|February 28, 2024

概括

这项研究增强了近红外II (NIR-II) 反射共聚焦显微镜与相对比,改善了深度大脑成像,并为神经科学研究提供了详细的血管映射.

科学领域:

神经科学是一个神经科学.
光学显微镜的使用方法
生物医学成像技术生物医学成像技术

背景情况:

显微镜成像深度受到光线透到生物组织的限制.
近红外II (NIR-II) 频谱与反射共聚焦显微镜相结合,可提供更高的成像深度.
现有的NIR-II系统需要对细胞分化进行光学和后处理改进.

研究的目的:

在NIR-II反射共聚焦显微镜中实现相位对比检测方案.
增强皮质细胞和小血管的分化.
开发用于动态信号采集和血管映射的方法.

主要方法:

将相对比检测方案集成到反射共焦显微镜中.
使用NIR-II光谱范围进行照明.
开发一种采集方法,以区分动态信号与背景噪声.

主要成果:

成功实施相对比用于深层皮层成像,高达[特定深度],使用头骨窗口.
在类似的深度生成血管图,可与光学连贯性断层扫描相提并论.
结合多个图像以创建全面的船舶网络的演示.

结论:

阶段对比反射共焦显微镜可以改善皮层细胞体的可视化.
提出的框架允许从动态生物信号中检索血管图.
这项工作改进了以前的显微镜设计,采用了新的光学实现和分析技术.

关键词:

在NIR-II显微镜中使用NIR-II显微镜皮层显微镜的研究.内在成像内部成像内部成像阶段对比显微镜阶段对比显微镜反射共聚焦显微镜反射共聚焦显微镜

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