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对mopox病毒F3L表达细胞的转录组和蛋白质组分析

Yihao Wang^1,2,3, Junzhe Zhang^1,2,3, Mingzhi Li^1,2,3

¹Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang, Jiangxi, China.

Frontiers in cellular and infection microbiology

|February 28, 2024

概括

莫波克斯病毒F3蛋白通过升级抗病毒基因来增强先天免疫反应,并与参与RNA拼接和翻译的宿主蛋白相互作用,提供有关莫波克斯病原学的见解.

科学领域:

病毒学病毒学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

麻疹病毒 (mpox) 构成了全球健康威胁.
尽管F3蛋白具有假定的RNA结合域,但其功能尚不清楚.
本研究使用转录组和蛋白组分析来研究F3L蛋白的作用.

研究的目的:

为了阐明mopox F3蛋白的功能.
识别与F3相互作用的宿主细胞蛋白质.
了解F3L对宿主基因表达和免疫通路的影响.

主要方法:

在F3L转移的HEK293T细胞中进行RNA测序 (RNA-seq).
蛋白质组学 (LC-MS/MS) 用于识别F3相互作用蛋白.
实时PCR验证差异表达基因.

主要成果:

通过RNA-seq鉴定出1672个差异表达基因 (DEGs),其中1156个是上调调的,516个是下调调的.
蛋白质组学确定了27种候选相互作用蛋白,主要涉及RNA剪接和翻译.
缩分析显示,免疫相关途径的显著缩,包括I型干扰素信号传递.

结论:

在F3L上调节关键的先天性免疫基因 (如RIGI,MDA5,IRF5,IFNA14),促进抗病毒反应.
F3蛋白与宿主蛋白相互作用,这对RNA拼接和翻译至关重要 (例如,SNRNP70,HNRNPA1).
这些发现有助于我们更好地了解在mopox病毒感染中F3蛋白的功能.

关键词:

F3L F3L 在线播放天生的免疫信号通路.莫波克斯病毒 (MPOX) 病毒蛋白质基质的保护性转录组 (transcriptome) 是一个转录组.

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