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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
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Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
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Cdc14酸酶,Clp1,不会影响基因组表达.

Luis Lopez Maury1,2, Liping Ren3, Shaimaa Hassan1

  • 1Department of Genetics, Evolution, and Environment, Institute of Healthy Ageing, University College London, London, UK.

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酸酶的Clp1不调节基因表达. 这突出了保存的酸酶家族.

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科学领域:

  • 细胞生物学 细胞生物学
  • 分子遗传学 分子遗传学
  • 生物化学 生物化学

背景情况:

  • 在 * Schizosaccharomyces pombe * 中的 Cdc14 族酸酶 Clp1 逆转了线粒体 Cdk1 酸化.
  • 哺乳动物中Clp1的ortologs具有不同的功能,包括在DNA修复和纤毛发育中的作用.
  • Clp1在基因调节中的确切作用尚不清楚.

研究的目的:

  • 为了调查 * Schizosaccharomyces pombe * Clp1 是否在调节基因表达方面发挥作用.
  • 要确定Clp1的功能是否与观察到的*clp1∆*细胞对LatrunculinA的敏感性有关.

主要方法:

  • 野生型和*clp1∆*细胞的比较转录概况.
  • 对抗激素脱聚合药物LatrunculinA.细胞反应的分析.

主要成果:

  • 发现Clp1对*Schizosaccharomyces pombe*的基因表达没有可检测的影响.
  • *clp1∆* 细胞对LatrunculinA的敏感性似乎没有被转录反应介导.

结论:

  • 在 *Schizosaccharomyces pombe* 中,Clp1 的功能与其更高的真核体正体不同.
  • 保存的酸酶家族表现出特定于生物体的功能多样化.