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利用具有成本效益的便携式设备来增强COVID-19变种在现场和大规模的跟踪

Sung Yong Park¹, Gina Faraci¹, Pamela Ward²

¹Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.

Journal of clinical microbiology

|February 28, 2024

概括

一个新的便携式工具在不到10小时内快速测序SARS-CoV-2 (严重急性呼吸系统综合征冠状病毒2) 变体. 这种具有成本效益的技术可以在偏远地区加强对COVID-19 (冠状病毒疾病2019) 的全球监测.

科学领域:

基因组监测是指基因组的监测.
传染病流行病学传染病流行病学
便携式测序技术是可以携带的.

背景情况:

全球对COVID-19 (冠状病毒疾病2019) 变种的基因组监测分布不均,来自低资源环境和土著社区的数据有限.
现有的用于病原体跟踪的便携式测序工具往往具有很长的样本到答案时间 (约. 24小时) 并需要外部电源和互联网连接.
由于生活条件偏远和独特的社会习惯,土著人口面临着不成比例的COVID-19风险.

研究的目的:

开发和评估一种便携式,具有成本效益的工具,用于对SARS-CoV-2 (严重急性呼吸系统综合征冠状病毒2) 变种的快速全基因组测序.
评估便携式测序系统的样本到答案的时间,准确性和吞吐量.
证明该工具在资源有限的环境和偏远地区进行大规模监控的实用性.

主要方法:

利用小型,易于使用和廉价的设备进行SARS-CoV-2 (严重急性呼吸系统综合征冠状病毒2) 全基因组测序.
在没有外部电源或互联网的情况下,使用独立的笔记本电脑进行了测序,基因组组装和血统确定.
将便携式测序方法的准确性与两个独立的测序技术进行了比较.

主要成果:

便携式系统在9小时15分钟内在感染者身上识别出SARS-CoV-2 (严重急性呼吸系统综合征冠状病毒2) 变种.
实现了序列运行,组装和血统确定时间仅为16分钟.
在一次运行中生成了289个SARS-CoV-2 (严重急性呼吸系统综合征冠状病毒2) 全基因组序列,使现有方法的吞吐量增加了三倍 (96个序列/运行).

结论:

开发的便携式测序方案灵活,快速,准确,在现有技术上提供了显著的进步.
这种工具对于低收入和中等收入国家的大规模监测以及偏远地区的有针对性的研究非常有价值.
该系统提高了追踪新出现的COVID-19 (冠状病毒疾病2019) 变种的能力,特别是在服务不足的人群中.

关键词:

这种COVID变种是COVID变种.这是下一代测序.便携式测序可以随身携带.

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