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一个真菌基因与细菌操作子的功能和进化整合

Liang Sun^1,2, Kyle T David³, John F Wolters^1,2

¹DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI 53726, USA.

Molecular biology and evolution

|February 28, 2024

概括

维克拉米埃拉/星梅雷拉酵母获得了用于合成肠杆菌素的细菌基因. 研究人员确定Enb1是关键的进口物,使铁吸收和适应低铁环境成为可能.

科学领域:

微生物学微生物学
酵母遗传学酵母遗传学
横向基因转移是指水平基因转移.

背景情况:

赛德罗对于微生物的铁获取至关重要.
大多数酵母不能合成 siderophores,但可以进口它们.
维克拉米拉/星梅拉 (W/S) 酵母通过横向基因转移独特地获得了肠杆菌素合成基因.

研究的目的:

为了确定W/S类酵母酵母中的肠杆菌素进口者.
了解酵母酵母中肠杆菌素吸收和合成的进化历史.
研究细菌和真菌基因的功能整合.

主要方法:

对ENB1和肠杆菌素合成基因的家族基因分析.
遗传学拓测试以推断基因转移事件.
在W/S类和Saccharomycetales之间进行比较基因组学.

主要成果:

Enb1被确定为Starmerella bombicola.的主要肠杆菌进口物.
在西南亚酵母中,ENB1的存在与肠杆菌素合成基因保留有关.
星菌 (Starmerella stellata) 是一种"骗子"酵母,它利用肠杆菌素而不产生它.
在Saccharomycetales中,ENB1可能早于肠杆菌素基因的获取,并经历了HGT.

结论:

菌类ENB1和细菌Ent基因在W/S酵母中形成一个合作单位,以获得铁.
ENB1和ent基因的进化动态解释了酵母肠杆菌素生产者和作弊者的分布.
这种综合系统强调了通过真菌-细菌基因协同作用来适应缺乏铁的环境.

关键词:

细菌是一种细菌.肠杆菌素是什么肠杆菌素这是一种真菌的真菌菌.铁是铁的,铁是铁的在Operon中使用.在 siderophore 的位置上.

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