通过Cre/loxP系统回收可选标记物,用于构建共同表达多种蛋白质的Komagataella phaffii菌株

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Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers

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Weixian Wang1, Minghai Han2, Guofei Zhu3

  • 1Analytical and Testing Center, Guizhou Institute of Technology, Guiyang, 550000, China.

Biotechnology letters
|February 28, 2024
PubMed
概括

本研究提出了一个简单的Cre/loxP系统,用于回收Komagataella phaffii酵母中的可选择标记物. 这种方法通过顺序基因整合,使多种蛋白质能够有效地共同表达.

关键词:
科玛格塔贝拉 (Komagataella phaffii) 是一个有趣的植物.这就是Pichia pastoris.同表达多种蛋白质.克雷洛克斯 (Cre/loxPP) 是一个植物.可以选择的标记.

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