一个圆形的RNA-gawky-chromatin调节轴调节压力诱导的转录
Rui Su1, Min Zhou2,3, Jiamei Lin1
1School of Life Sciences, Chongqing University, Chongqing 401331, China.
Nucleic acids research
|February 28, 2024
概括
响应金属的circRNAs通过将RNA结合蛋白在细胞质中隔离来抑制压力诱导的基因转录. 这一发现揭示了真核生物基因表达中的新型circRNA-RBP-染色体调节轴.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 在RNA生物学,RNA生物学.
背景情况:
- 重金属压力会触发细胞反应,包括核转移RNA结合蛋白 (RBP) 激活应激基因.
- 上游的调节者和控制古怪中介转录的精确机制在很大程度上仍未被阐明.
研究的目的:
- 为了识别重金属应力期间的Gawky介导转录的上游调节者.
- 阐明这些调节器调节的功能和基因表达的分子机制.
主要方法:
- 识别含有金属响应元素的circRNAs (MRE circRNAs) 与gawky相互作用.
- 使用Drosophila MT作为读取结果的MREcircRNAs的过度表达和淘汰研究.
- RNA测序 (RNA-seq) 来识别受影响的基因.
- 对gawky-chromatin相互作用和亚细胞局部化的分析.
主要成果:
- 在铜应力过程中,MRE环RNAs 特别与 gawky 相互作用.
- 过度表达MRE环RNAs抑制了压力诱导的转录基因,如Drosophila MT.
- MRE环RNAs会从染色质和细胞质积累中引起奇怪的解离,从而阻碍RNA聚合酶II的加载.
- 超过500个应激反应基因在MRE circRNA过度表达时显示了减弱的转录.
- MRE动机对于circRNA-gawky相互作用和抑制功能至关重要.
结论:
- 一个涉及circRNA-RBP-染色体相互作用的新型调节网络被提议用于基因表达控制.
- MRE circRNAs通过调节的活动,在抵抗铜过载的细胞防御中发挥着重要作用.
- 这项研究强调了circRNAs作为重金属应激反应和更广泛的真核细胞基因调节中的关键参与者.
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