淋巴结转移的调节通过周桃体组织线粒体相关途径的激活在口腔癌中的淋巴结转移
Naohiro Wakisaka1,2, Makiko Moriyama-Kita2, Satoru Kondo2
1Department of Otorhinolaryngology, NHO Kanazawa Medical Center, Kanazawa, Ishikawa, Japan.
PloS one
|February 28, 2024
概括
口腔癌 (OPC) 结节状态改变了桃体中的免疫基因表达. 与转移阳性病例相比,转移阴性OPC显示出不同的免疫细胞配置和线粒体代谢途径.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 基因组学就是基因组学.
背景情况:
- 口腔癌 (OPC) 的进展受到瘤微环境的影响.
- 免疫细胞透和周组织中的功能可以影响癌症的传播.
- 了解与OPC节点状况相关的桃体组织的免疫变化至关重要.
研究的目的:
- 根据OPC节点状况调查瘤周边桃体组织的免疫代谢和免疫细胞计数变化.
- 识别与线粒体代谢和免疫反应相关的差异表达基因 (DEG).
- 为了将这些变化与OPC患者转移的存在相关联.
主要方法:
- 用948基因的线粒体代谢基因组对27个周围口桃体组织进行微阵列分析.
- 生物信息分析包括REACTOME路径分析和CIBERSORTx用于免疫细胞比例估计.
- 数字空间分析和下一代测序用于线粒体DNA (mtDNA) 变体检测.
主要成果:
- 在转移阴性和转移阳性OPC病例之间确定了228个DEG,其中206个基因被上调.
- 在Toll类受体4级联通路中,上调基因被丰富.
- 转移阴性病例显示了较高的原始B细胞和较低的调节性T细胞以及休息的自然杀手细胞.
- 根据结节状况,数字空间分析显示了淋巴状区域的DEG,但不是瘤区域.
- 在OPC组织中没有发现显著的mtDNA变异.
结论:
- 周围瘤桃体免疫基因表达特征受到OPC节点状态的显著修改.
- 与线粒体新陈代谢相关的独特的转录程序和免疫细胞简介与OPC淋巴传播有关.
- 这些发现凸显了OPC淋巴扩散中潜在的向性免疫机制.
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