来自粘液的蛋白质冠状体作为矛和盾牌来调节肠细胞中转移素功能化纳米粒子跨细胞运输的作用
Dan Yang1,2, Yuqi Feng1, Ying Yuan1
1School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an, Shaanxi 710021, China.
ACS nano
|February 28, 2024
概括
粘液通过形成蛋白质冠状体 (PCs) 来阻碍纳米粒子 (NP) 传递,但用粘素预涂层NP可增强细胞吸收和运输,从而提高口服纳米药物的疗效.
科学领域:
- 纳米医学是一种纳米医学.
- 生物材料科学 生物材料科学
- 胃肠道药物输送 胃肠道药物输送
背景情况:
- 表皮粘膜对通过纳米颗粒 (NP) 传递药物构成重大障碍.
- 蛋白冠状体 (PCs) 在与生物流体相互作用时在NP上形成,改变它们的行为.
- 有限的研究存在于PCs形成在上皮膜粘膜接口,特别是与粘液.
研究的目的:
- 研究表皮粘膜相互作用对PC形成和转移素修饰NP (Tf-NP) 细胞间传输的影响.
- 探索克服粘液诱导的纳米药物交付障碍的策略.
主要方法:
- 作为一个模型系统,构建了转激素修饰的NP (Tf-NP).
- 研究了PC的形成及其对Tf-NP在分泌粘液和无粘液细胞中的细胞间传输的影响.
- 开发了先前涂有粘素的Tf-NP (M@Tf-NP),以减轻PC的负面影响.
主要成果:
- Tf-NPs从粘液中吸附了更多的蛋白质,掩盖了向配体,并减少了内细胞分裂和运输.
- 在没有粘液的细胞中,NP在细胞内吸附蛋白质,增强转细胞形成.
- M@Tf-NPs显示减少了负面PC影响,增强了内细胞分裂,并通过ER-Golgi通路改善了细胞内传输.
结论:
- 粘液可以阻碍NP的吸收,但也可以利用它来增强上皮屏障的透.
- 通过调节PC形成和增强细胞运输,提供了一种改善口服纳米药物输送的策略.
- 这些发现为设计用于口服的先进纳米药物提供了基础.
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