使用可切换pH的水友性溶剂从人体尿样中微提取克洛米普拉
Maria Terzi1, Natalia Manousi2, Paraskevas D Tzanavaras2
1Laboratory of Pharmaceutical Analysis, Department of Pharmacy, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
概括
一种新型的pH可切换的水友性溶剂 (SHS) 方法有效地从尿液中提取克洛米普拉 (CLP). 这种具有成本效益和绿色分析方法简化了临床和法医环境中的药物度监测.
科学领域:
- 分析化学 分析化学
- 环境化学环境化学
背景情况:
- 准确的克洛米普拉 (CLP) 的确定,三环抗抑郁药,对于临床和法医评估至关重要.
- 在生物矩阵中确定CLP的现有方法可能是复杂和耗时的.
研究的目的:
- 开发和验证一种新的,高效的,绿色的微提取方法,用于人类尿中的克洛米普拉 (CLP).
- 为了利用酸作为pH可切换的水友性溶剂 (SHS) 来增强CLP提取.
主要方法:
- 从人类尿液中微提取CLP,使用酸作为可切换pH的水友性溶剂 (SHS).
- 由pH值变化引起的SHS的相变为快速分散和分离.
- 使用高性能液体色谱-紫外线检测 (HPLC-UV) 确定CLP.
主要成果:
- 优化的SHS微提取方法表现出良好的线性 (0.055.0μg mL-1),检测极限低 (0.015μg mL-1),量化极限低 (0.05μg mL-1).
- 实现了高准确度和精度,相对恢复值在90.0110.0%之间,RSD值低于9.1%.
- 该方法得到了验证,并显示了成本效益,简单性,快速性和绿色分析配置文件.
结论:
- 拟议的SHS微提取方法为尿液中CLP的确定提供了一种经济有效,快速和环保的方法.
- 这种方法提高了样品吞吐量,并证明了其在常规临床和法医分析中具有很好的适用性.
更多相关视频
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
9.6K
09:01Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
Published on: June 26, 2020
7.0K
相关概念视频
01:05High-Performance Liquid Chromatography: Elution Process
477
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
477
00:56Extraction: Advanced Methods
446
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
446