子油体的结构特征和稳定性分析及其用于加载β-胡卜素的应用
Yan Tian1, Xinxin Zhao1, Zhiguo Wang1
1School of Food Science and Engineering, Hainan University, Hainan 570228, China.
Food chemistry
|February 28, 2024
概括
子油体 (COB) 有效地加载β-胡卜素 (β-CA),提高其生物可访问性. 在性pH下,COBs表现出稳定性,但对离子强度和热量敏感.
科学领域:
- 食品科学与技术 食品科学与技术
- 营养药物输送系统 营养药物输送系统
- 生物材料科学 生物材料科学
背景情况:
- 子油体 (COB) 是天然的脂蛋白结构,具有封装生物活性化合物的潜力.
- 了解COB的结构特征和稳定性对于开发有效的交付系统至关重要.
- β-胡卜素 (β-CA) 是一种具有有限生物可用性的重要营养素,需要改进的输送方法.
研究的目的:
- 研究子油体 (COB) 的结构性质和稳定性.
- 评估COBs在加载和保留β-胡卜素 (β-CA) 的有效性.
- 评估COB结构和稳定性对β-CA生物可访问性的影响.
主要方法:
- 使用SDS-PAGE.COB成分 (脂质,蛋白质,水分) 的表征.
- 通过动态光散射分析COB滴粒大小和分布.
- 在不同的pH值,离子强度和热条件下评估COB的稳定性.
- 用自由脂肪酸分析和模拟胃肠道消化来评估β-CA加载和释放.
主要成果:
- COBs主要由中性脂质 (81.1%) 和膜蛋白 (0.6%) 组成,分子重量为12-40kDa.
- COBs具有小滴径 (5.1微米) 和单模分布,在性pH (8-10) 显示稳定性.
- COBs在离子强度和热处理方面表现不稳定,但有效地加载和保留β-CA,提高其生物可访问性.
结论:
- 子油体是适合装载β-CA.等生物活性化合物的矩阵.
- 优化COB稳定性是最大限度地提供营养药的关键.
- 这项研究支持使用COB来增强有价值营养素的生物可用性.
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