保存的抗原诱导呼吸道Th17介导的广泛血清型保护,防止肺炎球菌超级感染
Xue Liu1, Laurye Van Maele2, Laura Matarazzo2
1Department of Pathogen Biology, Base for International Science and Technology Cooperation, Carson Cancer Stem Cell Vaccines R&D Center, International Cancer Center, Shenzhen University Medical School, Shenzhen 518060, China; Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Biophore Building, CH-1015 Lausanne, Switzerland.
一种新的通用肺炎球菌疫苗抗原LafB,即使在流感感染后,也显示出高效率. 用LafB进行呼吸道疫苗接种可以保护肺炎链球菌,提供了一个有前途的新疫苗策略.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
背景情况:
- 细菌疫苗在病毒共感染期间经常失效,需要新的疫苗方法.
- 肺炎链球菌对健康造成重大负担,现有的疫苗存在局限性.
研究的目的:
- 为了识别流感感染后有效的新型Streptococcus pneumoniae抗原.
- 评估保存的lafB基因作为通用肺炎球菌疫苗标的潜力.
主要方法:
- 在小鼠超级感染模型中进行CRISPR干扰测序 (CRISPRi-seq).
- 描述LafB蛋白及其在细菌细胞壁恒温中的作用.
- 评估小鼠用复合LafB进行的呼吸道与皮下疫苗接种.
主要成果:
- 鉴定出 lafB 基因对 S. pneumoniae 毒性至关重要.
- 通过呼吸道疫苗接种的重组LafB给予了对小鼠多种S. pneumoniae血清型的保护.
- 保护是由CD4+ T辅助细胞17介导的,独立于抗LafB抗体.
- 在健康的人类个体中,LafB表现出抗原性.
结论:
- 拉夫B是一种有前途的通用肺炎球菌疫苗抗原,即使在流感感染后也有效.
- 针对LafB的呼吸道疫苗接种提供了针对S. pneumoniae的新策略.
- 这些发现表明,对于容易与病毒共感染的细菌病原体,疫苗学是一种新的方法.
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