相关实验视频

Updated: Jul 2, 2025

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Published on: March 14, 2014

通过LCP1介导的细胞骨变化涉及人类不朽的前列腺层细胞的酸盐触发的恶性表型

Yiping Yang¹, Menghan Zhou¹, Yurun Huang¹

¹Department of Research, Guangxi Medical University Cancer Hospital, Nanning, 530021, China.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

|February 28, 2024

概括

持续的低剂量暴露会导致前列腺细胞变得恶性. 这涉及细胞结构的变化和通过ROS-ATM-ERK1/2途径的迁移,为前列腺癌提供潜在的治疗点.

科学领域:

环境毒理学环境毒理学
癌症生物学癌症生物学
分子机制的分子机制

背景情况:

暴露和前列腺癌之间确立的联系.
诱导的瘤发生机制尚不清楚.

研究的目的:

在持续低剂量化物暴露下调查前列腺 stromal 细胞 (WPMY-1) 的恶性转变.
阐明了亚石诱导的前列腺癌进展背后的分子机制.

主要方法:

持续暴露WPMY-1细胞在1和2μM化物中29周.
定量蛋白质组学用于识别差异表达蛋白 (DEP).
细胞实验评估细胞骨,活性氧物种 (ROS) 和信号通路 (ATM,ERK1/2).

主要成果:

化物暴露诱导了WPMY-1细胞中的恶性表型.
蛋白质组学确定了517个DEP,突出了细胞骨,细胞粘附和迁移的变化.
化物暴露增加了ROS,激活了ATM和ERK1/2信号,并上调了LCP1蛋白,影响了F-actin组织.

结论:

ROS-ATM-ERK1/2信号通路介导LCP1的激活,导致细胞骨的改变.
这些变化有助于酸盐诱导的瘤微环境细胞的恶性表型.
研究结果表明,与有关的前列腺癌的潜在生物标志物和治疗策略.

关键词:

亚是一种.细胞骨架细胞骨架在LCP1中,LCP1是指LCP1.恶性现象型的恶性现象型.在WPMY-1细胞中.

更多相关视频

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

Published on: September 20, 2016

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

Published on: March 6, 2018

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...