相关实验视频

Updated: May 5, 2026

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

适配蛋白HIP-55通过促进AP-1复合体激活促进巨细胞M1极化

Jingwei Bian¹, Yuzhong Zhu², Panhui Tian³

¹Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital; Beijing Key Laboratory of Cardiovascular Receptors Research; Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Ministry of Health; State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing 100191, China.

Cellular signalling

|February 28, 2024

概括

适配蛋白HIP-55关键调节巨细胞M1两极分化,这是心血管疾病中的一个关键过程,由氨酸-血管酶-氨酸系统 (RAAS) 功能障碍驱动. 通过激活AP-1复合体,HIP-55促进了这种两极分化.

科学领域:

免疫学免疫学免疫学
心血管生物学心血管生物学
分子医学是分子医学.

背景情况:

巨细胞M1两极分化是由氨酸-血管酶-阿尔多素系统 (RAAS) 功能障碍驱动的,在心血管疾病中促进炎症.
管理这种M1两极分化的精确监管机制仍然不完全理解.

研究的目的:

在RAAS功能障碍的背景下,识别巨细胞M1两极分化的关键调节者.
阐明HIP-55影响Ang II诱导的M1偏振的分子机制.

主要方法:

研究了Ang II刺激的M1巨细胞中的HIP-55表达.
在巨细胞极化研究中利用了HIP-55的过度表达和遗传删除.
研究了HIP-55对激活剂蛋白-1 (AP-1) 复合体激活的影响,包括ERK1/2和JNK酸化.
评估了阻断AP-1激活对HIP-55在巨分化中的功能的影响.

主要成果:

在Ang II诱导的M1巨细胞中,HIP-55的表达被上调.
HIP-55的过度表达增强了Ang II诱导的M1极化,而HIP-55的删除则抑制了它.
HIP-55通过增强ERK1/2和JNK酸化来促进Ang II诱导的AP-1激活.
抑制AP-1激活可以抵消HIP-55对巨细胞两极分化的影响.

结论:

HIP-55作为巨细胞M1极化的一个关键调节器.
HIP-55通过AP-1信号通路调解其影响.
这些发现为与RAAS功能障碍相关的心血管疾病提供了潜在的治疗策略.

关键词:

激活蛋白-1-1是一种激活蛋白.在HIP-55的基础上,HIP-55是HIP-55.在M1极化中,M1极化巨细胞是一个巨细胞.

更多相关视频

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Published on: August 2, 2021

相关概念视频

Antigen Presenting Cells

Antigen Presenting Cells

The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...