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mm9_circ_014683通过糖尿病视网膜病变的正规NFκB信号通路调节微质极化

Tingting Chen¹, Congyao Wang¹, Wenhui Zhu¹

¹Department of Ophthalmology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.

Cellular signalling

|February 28, 2024

概括

循环RNAs调节糖尿病视网膜病变 (DR) 中的微质极化. 这项研究确定了一个关键的circRNA,mm9_circ_014683,它通过NFκB通路影响M1/M2极化,为DR提供了潜在的治疗点.

科学领域:

眼科医生眼科眼科
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

糖尿病视网膜病变 (DR) 导致显著的视力丧失,视网膜微质介导的炎症是关键因素.
以前的研究表明,M1微质极化有助于DR炎症,但潜在的机制需要进一步阐明.
循环RNAs (circRNAs) 正在成为细胞过程中的关键调节者.

研究的目的:

研究circRNAs在高葡萄糖诱导的微质激活中的作用.
在DR的背景下,确定参与微质偏振的特定circRNAs.
探索已识别的circRNAs调节微质功能的分子机制及其与DR病原发生的相关性.

主要方法:

在暴露于高葡萄糖的BV2小鼠微质体中的差异性circRNA表达概况.
功能性试验评估circRNA mm9_circ_014683对微质增殖,亡和极化 (M1/M2) 的影响.
在circRNA-knockdown细胞中分析基因表达和通路丰富 (NFκB信号);在糖尿病小鼠模型中的体内验证.

主要成果:

在用葡萄糖治疗的BV2细胞中鉴定出347个差异表达的circRNAs,其中mm9_circ_014683被显著上调.
操纵mm9_circ_014683水平调制M1/M2微质偏振,而不影响增殖或亡.
发现mm9_circ_014683能积极调节正规NFκB信号通路,并且在糖尿病小鼠的视网膜微质中表达高.

结论:

在糖尿病视网膜病变中,circRNA mm9_circ_014683在调节微质极化方面发挥着至关重要的作用.
这种调节通过正规的NFκB信号通路发生.
mm9_circ_014683 是一种潜在的治疗点,用于治疗DR相关的炎症和视力丧失.

关键词:

糖尿病视网膜病变 - 糖尿病视网膜病变微质质的两极分化在NFκB信号通路中.环RNA 环RNA 是一个环RNA.

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