一种多用途的黄毒素的结构,动力学和氧化还原活性
Sharique Khan1, Ahmadullah Ansari2, Monica Brachi3
1Department of Chemistry, University of Kentucky, Lexington, Kentucky, USA.
The Journal of biological chemistry
|February 28, 2024
概括
来自Rhodopseudomonas palustris (Rp9Fld) 的黄毒素转移电子用于重要过程. 它的结构和动力学,使用19F核磁共振研究,表明参与合作伙伴结合和电子转移的灵活循环.
科学领域:
- 生物化学 生化学
- 结构生物学 结构生物学
- 微生物学 微生物学
背景情况:
- 黄素 (Fld) 是微生物新陈代谢必不可少的电子载体.
- Rhodopseudomonas palustris CGA009 flavodoxin (Rp9Fld) 提供了酶的降解等价物,特别是在铁的限制下.
- 光驱动的电子流通过分叉电子传输蛋白力量Rp9Fld.
研究的目的:
- 阐明Rp9Fld与各种蛋白质合作伙伴相互作用的结构基础.
- 使用19F NMR. 调查Rp9Fld的动力学和形状灵活性.
- 了解氨酸残留物在Rp9Fld的功能和氧化还原特性中的作用.
主要方法:
- 进行X射线晶体学以确定Rp9Fld的三维结构.
- 19F核磁共振 (NMR) 光谱学用于分配和分析氨酸信号.
- 电化学测量以评估氧化还原性质并探测黄-氨酸相互作用.
主要成果:
- Rp9Fld 呈现了一种正规的 Fld 折叠,带有一些可变的表面环.
- 19F核磁共振和电化学数据显示了flavin和伴侣结合区域附近的循环中的动态.
- 氨酸的修改对氧化还原特性产生了轻微影响,但证实了与flavin半农素的接近.
结论:
- Rp9Fld的表面环在形状上是灵活的,这表明在合作伙伴结合中具有适应性.
- 循环动态和保护模式表明功能适应性,而不是严格的合作伙伴特异性.
- 在伴侣结合时改变的环形状可能会积极参与电子转移.
关键词:
(19) F NMR 的 NMR 是什么?在X射线中,晶体结构是X射线.有动态循环的循环.动力学 动力学 动力学电子转移是电子的转移.黄毒素 (flavodoxin) 是一种可怕的药物.表面循环环的循环.更多相关视频
08:57Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
10.1K
08:31Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
8.5K
相关概念视频
Oxidation of Phenols to Quinones
3.0K
In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
3.0K
Redox Equilibria: Overview
564
A reduction-oxidation reaction is commonly called a redox reaction. In a redox reaction, electrons are transferred from one species to another rather than being shared between or among atoms. The reducing agent or reductant is the species that loses electrons and gets oxidized in the process. The species that gains electrons and gets reduced in the process is the oxidizing agent or oxidant. Redox reactions are represented as two separate equations called half-reactions, where one equation...
564
Role of Reduced Coenzymes NADH and FADH₂
11.5K
The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
11.5K
Redox Reactions
55.6K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
55.6K
Protein Dynamics in Living Cells
2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Oxidation-Reduction Reactions
64.9K
Oxidation–Reduction Reactions
64.9K
