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主体miRNAs和circRNAs和ceRNA网络在Toxoplasma gondii溶解周期期间的表达特征

Sha-Sha Wang¹, Xiangwei Wang¹, Jun-Jun He²

¹State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.

Parasitology research

|February 28, 2024

概括

这项研究揭示了微RNA (miRNA) 和循环RNA (circRNA) 在东道细胞中在Toxoplasma gondii感染期间如何发生变化的新见解. 这些非编码RNA涉及至关重要的细胞过程,如免疫反应和亡.

科学领域:

寄生虫学的寄生虫学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

毒素菌是一种常见的寄生虫,会引起健康问题,特别是在弱势群体中.
非编码RNAs,包括microRNAs (miRNAs) 和循环RNAs (circRNAs),是感染中的关键调节者.
不同表达的miRNAs和circRNAs在宿主对T. gondii的溶解周期的反应中的特定作用仍然不清楚.

研究的目的:

在T. gondii感染后,研究人类前皮纤维细胞 (HFF) 中miRNAs和circRNAs的表达特征.
在感染后的不同时间点识别差异表达的miRNAs (DEmiRNAs) 和circRNAs (DEcircRNAs).
了解这些非编码RNA在宿主细胞反应中的功能影响.

主要方法:

RNA测序 (RNA-seq) 用于在HFF中描述miRNA和circRNA的表达.
感染时间点在感染后1.5至48小时之间 (hpi).
对差异表达的转录进行了生物信息分析,包括基因本体学和KEGG通路丰富.

主要成果:

在所有测试时间点中,发现了大量的DEmiRNA和DEcircRNA.
DEcircRNAs的数量显著增加,达到24hpi的峰值.
丰富分析表明,DE转录与免疫反应,亡,信号转导和新陈代谢途径有关.

结论:

这项研究提供了miRNA和circRNA在T. gondii感染期间表达变化的全面概况.
已识别的DEmiRNAs和DEcircRNAs参与调节宿主细胞反应,包括免疫逃避和细胞防御机制.
这些发现增强了我们对宿主-寄生虫相互作用背后的分子机制的理解,并为治疗干预提供了潜在的目标.

关键词:

这种毒性淋巴细胞 (Toxoplasma gondii) 是宿主病原体相互作用免疫反应的免疫反应.在RNA-seqqq.环RNA 环RNA 是一个环RNA.这是一个小RNARNA.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...