RNA螺旋酶DEAD-box-5参与了植入前胚胎的R循环动态
Hyeonji Lee1, Dong Wook Han2, Seonho Yoo1
1Department of Stem Cell and Regenerative Biotechnology, Institute of Advanced Regenerative Science, Konkuk University, Seoul 05029, Korea.
Animal bioscience
|February 29, 2024
概括
研究人员确定了RNA螺旋酶DDX5作为DNA的关键调节者:在小鼠胚胎早期发育过程中RNA R-循环动力学,影响胚胎基因激活和R-循环积累.
科学领域:
- 分子生物学分子生物学
- 发展生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- R环,DNA:RNA三重结构,对于基因转录至关重要,并且在小鼠囊中被动态调节.
- 了解R循环调节对于破译早期胚胎发育和胚胎基因激活至关重要.
- 在受精的小鼠卵中控制R循环稳态的因素在很大程度上仍未得到研究.
研究的目的:
- 识别和研究在小鼠胚胎中胚胎基因激活过程中调节R循环动态的候选因子的功能.
- 阐明在R循环代谢中特定蛋白质,如DEAD-box-5 (DDX5) 和胰岛素脱乙酶-2 (HDAC2) 的作用.
主要方法:
- 利用公开可用的下一代测序数据,包括核糖体分析和RNA聚合酶IIChIP-seq,用于候选物体的识别.
- 分析了质谱数据与测序数据集一起,以确定R循环动态的调节器.
- 使用化学抑制剂在体外受精的小鼠胚胎和通过免疫光学量化R-循环水平.
主要成果:
- 确定了DEAD-box-5 (DDX5) 和基因素脱乙酶-2 (HDAC2) 作为卵细胞,胚胎和双细胞胚胎R循环代谢的潜在调节剂.
- 抑制DDX5活动显著降低了前核中的R循环积累,证实了它在R循环动态中的作用.
- 抑制HDAC2活性并没有对前核中的R环水平产生显著影响.
结论:
- 鼠标囊中的动态R-循环变化很可能是由RNA基酶,特别是DDX5,以及转录调节的协调.
- 在胚胎早期发育过程中,DDX5在调节R循环动态方面发挥着重要作用.
- 这项研究提供了关键的洞察力,了解了关键的胚胎阶段控制R循环稳态的分子机制.
更多相关视频
相关概念视频
02:37Restarting Stalled Replication Forks
5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
00:55DNA Helicases
21.3K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
21.3K
03:01The Replisome
33.5K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.5K
02:41Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K
01:53Bacterial Transcription
28.2K
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
28.2K
02:35Transcription Elongation Factors
10.9K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
10.9K