人类患者的心房动与V型原蛋白和TGFbeta1增加有关
Sawa Kostin1, Manfred Richter2, Natalia Ganceva3
1Faculty of Health Sciences Brandenburg, Brandenburg Medical School Theodor Fontane, Neuruppin, Germany.
International journal of cardiology. Heart & vasculature
|February 29, 2024
概括
与心房动 (AF) 相关的心房纤维化,涉及增加的V原和TGF-β1.1. 这项研究在AF患者,特别是慢性AF患者中发现了更高的V原和纤维细胞标记物,突出了它们在AF病变发生中的作用.
科学领域:
- 心血管生物学 心血管生物学
- 连接组织研究 连接组织研究
- 心脏电生理学 心脏电生理学
背景情况:
- 心房纤维化是心房动 (AF) 开始和进展的关键因素.
- 原V是一种纤维原,在AF的背景下以前没有被研究过.
- 了解心房纤维化分子基础对于开发针对性治疗AF至关重要.
研究的目的:
- 在患有鼻节律 (SR) 和心房动 (AF) 的患者中调查V原蛋白的数量.
- 检查SR和AF中V原体,纤维细胞数量和TGF-β1表达之间的关系.
- 为了区分这些关系,需要区分 Paroxysmal AF (pAF) 和慢性,长期持久性AF (cAF).
主要方法:
- 在SR,pAF和cAF患者的心房活检中使用了定量免疫组织化学.
- 使用免疫组织化学测量V原蛋白水平.
- 纤维细胞计数 (维门丁阳性细胞) 和TGF-β1表达量化.
主要成果:
- 与SR相比,V原蛋白在pAF (1.8倍) 和cAF (3.1倍) 中显著增加.
- 纤维细胞数量 (vimentin阳性细胞) 在pAF (1.46倍) 和cAF (1.8倍) 与SR相比增加.
- 在pAF和cAF中,TGF-β1表达和阳性细胞计数显著增加,cAF的升高更大.
结论:
- 这项研究提供了第一个证据,将增加的V原蛋白和TGF-β1表达与AF病原和心房纤维化联系起来.
- 较高的V原水平与纤维细胞数量增加和TGF-β1.1相关.
- 观察到的V原蛋白,纤维细胞和TGF-β1的增加在慢性AF中比 Paroxysmal AF更明显.
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