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使用CRISPR/Cas9系统的大豆PEAPOD基因的局部定向突变发生改变了组织发育过渡

Jaechol Sim¹, Yuhei Kanazashi¹, Tetsuya Yamada¹

¹Graduate School of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.

Plant biotechnology (Tokyo, Japan)

|February 29, 2024

概括

大豆PEAPOD (PPD) 基因淘汰突变显示增长延迟和保持绿色的表型. 失去PPD功能会影响SPIRAL1-LIKE 5的表达,影响大豆的器官生成,导致的异常发育.

科学领域:

植物生物学植物生物学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

植物器官的大小是由细胞数量和扩张调节的.
之前的工作使用CRISPR/Cas9.9生成了PEAPOD (PPD) 基因GmPPD1和GmPPD2的大豆突变.
一些突变者表现出异常的表型,如扭曲的和减少的种子,由于移突变.

研究的目的:

描述双倍PPD基因淘汰 (ppd-KO) 的大豆突变体的生理和分子特性.
调查ppd-KO大豆植物中观察到的异常表型的遗传基础.
探索PPD基因与大豆中SPIRAL1-LIKE 5 (SP1L5) 表达之间的关系.

主要方法:

在CRISPR/Cas9基因编辑中创建大豆ppd-KO突变体.
突变植物的表型分析,包括生长阶段和器官形态.
基因表达分析以评估PPD淘汰对GmSP1L5表达的影响.

主要成果:

这种ppd-KO突变体表现出延迟的植物生长和常绿色的表型,与其他大豆突变体不同.
在ppd-KO突变体中观察到SPIRAL1-LIKE 5 (GmSP1L5) 的显著减少表达.
在PPD基因功能和SP1L5表达之间建立了一个新的联系,解释了扭曲的表型.

结论:

完全淘汰PPD基因会对大豆器官产生产生负面影响.
减少PPD功能有助于大豆器官扩大.
PPD-SP1L5调节途径对于大豆正常发育和器官形成至关重要.

关键词:

这就是CRISPR/Cas9的作用.甘氨酸最大值 (Glycine max) 是一种皮阿波德 (Peapod) 是一种小鸟.螺旋1 - 类似于5 器官扩大器官扩大

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CRISPR

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