交替的磁场通过生成活性氧物种来驱动基因表达的刺激
Jordan W Mundell1,2, Matthew I Brier1,2, Everest Orloff1
1Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
iScience
|February 29, 2024
概括
磁遗传学使用无线电频率交替磁场来控制细胞. 这项研究表明,TRPV1通道的依赖费里的活性氧物种 (ROS) 激活启动了这种细胞反应.
科学领域:
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 磁遗传学使细胞功能的远程控制成为可能.
- 反应性氧物种 (ROS) 参与启动下游信号通路.
研究的目的:
- 在磁遗传学平台中阐明射频交替磁场 (RF-AMF) 刺激的机制.
- 调查TRPV1-ferritin相互作用和ROS生成在细胞对RF-AMF反应中的作用.
主要方法:
- 利用化学生物学方法研究表达TRPV1-ferritin的HEK293T细胞的RF-AMF刺激.
- 测量了细胞内和细胞外ROS水平.
- 引入了TRPV1中氨酸残留的突变,并使用了无铁素.
- 使用特定的抑制剂来识别信号通路组件.
主要成果:
- 射频-AMF刺激使细胞内和细胞外ROS水平分别增加了约30%和140%.
- TRPV1对ROS敏感的氨酸残留物中的突变取消了RF-AMF诱导的流和下游基因转录.
- 破坏费里与TRPV1的结合也消除了反应.
- 信号传递涉及蛋白质激酶C,NADPH氧化酶和内质网膜.
结论:
- 对于启动磁遗传学来说,TRPV1的依赖费里的ROS激活至关重要.
- 这个机制为磁遗传学的潜在医学和生物技术应用提供了洞察力.
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