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在剪切压力内皮细胞的表观遗传变化

Thaís Silva Pinto¹, Geórgia da Silva Feltran¹, Célio Júnior da C Fernandes¹

¹Lab. of Bioassays and Cellular Dynamics, Department of Chemical and Biological Sciences, Institute of Biosciences, Paulista State University-UNESP, Botucatu, São Paulo, Brazil.

Cell biology international

|February 29, 2024

概括

高血流剪切应激改变了内皮细胞表观遗传学,减少了关键的组织蛋白标记并影响了HOXA13基因表达. 这揭示了对血管生物学和高血压的新见解.

科学领域:

血管生物学血管生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
机械生物学机械生物学

背景情况:

内皮细胞的表型是通过表观遗传变化,特别是基因组修饰来调节的,以应对血液流动的力量.
在剪切应力下控制内皮细胞行为的特定表观遗传机制尚未得到充分理解.

研究的目的:

在不同剪切应力条件下研究内皮细胞的表观遗传修饰.
阐明基因组修饰和基因表达变化的作用,以应对模拟的高血压力.

主要方法:

在体外实验中使用人类静脉内皮细胞.
将细胞置于受控的张力作用下,模拟正常血压和高血压的血流.
评估表观遗传标记 (H3K4ac,H3K27ac),基因脱乙酶 (HDAC) 蛋白水平,以及基因/lncRNA表达 (HOXA13,HOTTIP).

主要成果:

高剪压导致H3K4ac和H3K27ac表观遗传标记的减少.
观察到基因素脱乙酶 (HDAC) 蛋白水平的显著变化.
增加的剪切应力负面调节HOXA13基因表达和增加HOTTIP长非编码RNA表达.

结论:

这项研究提供了第一个证据,将基质子修饰和染色质紧缩与在高剪压下内皮细胞机制信号联系起来.
这些发现突出了HOXA13在血管生物学和高血压中的作用,表明了潜在的治疗点.
结果为开发小分子开辟了道路,以调节高血压条件下的表观遗传机制.

关键词:

霍蒂普 (HOTTIP) 是一个热门的酒店.在HOXA13中,我们可以看到HOXA13.细胞内皮细胞的内皮细胞.表观遗传学是指表观遗传学.这种高血压,高血压.剪切压力的压力.

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