识别和功能性表征保存的ciss-regulatory元素,负责菜作物的早期水果发育
Hongjia Xin1,2, Xin Liu1,3, Sen Chai2
1State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
The Plant cell
|February 29, 2024
概括
黄瓜作物中保存的非编码序列 (CNS) 调节水果的发育. 识别这些 cis 调节元件 (CREs) 提供了作物改进的目标和对植物进化的洞察力.
科学领域:
- 植物基因组学 植物基因组学
- 进化生物学是进化的生物学.
- 发育生物学是发展生物学.
背景情况:
- 在物种之间保存的Cis-regulatory元素 (CREs) 驱动表型变异.
- 黄瓜作物 (例如黄瓜,西瓜) 分享来自下层卵巢的水果发育过程.
- 保守的调节序列在黄瓜果子发育中的作用在很大程度上是未被探索的.
研究的目的:
- 在菜作物中识别和表征保存的非编码序列 (CNS).
- 研究这些中枢神经系统在调节水果发育中的作用.
- 为改善菜作物特征提供目标.
主要方法:
- 比较基因组学以确定六种虫物种中保存的非编码序列 (CNS).
- 可访问色素区域 (ACR) 和基因表达的全基因组概况.
- 集成CNS和ACR数据以确定监管要素.
- 使用CRISPR-Cas9基因编辑来验证候选中枢神经系统的功能.
主要成果:
- 确定了392,438个CNS,其中82,756个是特异性的.
- 绘制了20,86543,204个可访问的染色体区域 (ACR) 和它们的目标基因.
- 在所有六种物种中发现了1687个甲虫特异性的中枢神经系统与ACR重叠,可能调节757个基因.
- 两个中枢神经系统的CRISPR突变改变了水果的形状和NAC1和EXT类基因的表达.
结论:
- 保存的 cis 调节元件 (CREs),特别是虫特有的元素,在水果发育中起着至关重要的作用.
- 这些发现为增强菜作物特征提供了有价值的CRE目标.
- 该研究提供了关于CREs在植物发育中的进化作用的见解.
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