相关实验视频

Updated: Jul 2, 2025

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

Published on: June 19, 2012

病毒PIC-pocketing:RSV将转化预启动复合体绑定到双相生物分子凝聚物中

Fatoumatta Jobe¹, James T Kelly¹, Jennifer Simpson¹

¹The Pirbright Institute, Woking, Surrey, United Kingdom.

Journal of virology

|February 29, 2024

概括

呼吸道同胞性病毒 (RSV) 通过创建称为包容体 (IBs) 的专门隔间来劫持宿主细胞,以增强病毒mRNA翻译. 这些IB进一步被组织成不同的阶段,集中转化机制以实现有效的病毒复制.

科学领域:

病毒学病毒学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

脊髓肺炎病毒,包括呼吸道同胞病毒 (RSV),形成细胞质包容体 (IBs),对病毒RNA复制和转录至关重要.
RSV感染导致人类和奶牛的显著发病率和死亡率,需要对其复制机制有更深入的了解.

研究的目的:

调查RSV纳入机构 (IBs) 的结构组织和功能细分.
确定RSV如何操纵IBs内的宿主细胞的翻译机制,以有利于病毒mRNA翻译.
确定抗病毒药物开发的潜在治疗点.

主要方法:

相对光和电子显微镜 (CLEM) 可视化IB结构.
光in situ杂交 (FISH) 来确定病毒mRNA的局部化.
利波基化试验用于评估活性翻译.
质谱法用于分析宿主蛋白质的修饰.

主要成果:

RSV IBs表现出具有独特功能域 (包括体相关颗粒,IBAGs) 的双相组织.
IBAG集中病毒mRNA,M2-1蛋白,以及关键的真核转化启动因子 (eIF4F,eIF3) 和40S核糖体复合体.
虽然大多数病毒转化发生在细胞质中,但证据表明一些IBAG内转化,表明分隔蛋白质合成.
病毒M2-1蛋白与eIF4G相互作用,促进IB阶段内的传输.

结论:

RSV IBs充当复杂的病毒器官,通过双相生物分子凝聚物空间调节病毒转化.
这种"PIC-pocketing"策略提高了病毒mRNA翻译和复制的效率.
了解这种机制可以了解病毒劫持宿主细胞机械和潜在的抗病毒点.

关键词:

RNA病毒的RNA病毒这就是为什么RSV RSV.基因表达的基因表达方式包容性组织是包容性机构.液体有机细胞液体有机细胞阶段分离的阶段分离.肺炎病毒的病毒.病毒翻译病毒翻译

更多相关视频

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

相关概念视频

Post-translational Translocation of Proteins to the RER

Post-translational Translocation of Proteins to the RER

A sizable fraction of proteins destined for ER are first synthesized in the cell cytosol and then transported across the ER membrane–a process called post-translational translocation. Similar to cotranslationally translocated proteins, these proteins also use the Sec translocon complex to enter the ER lumen.
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...

Directing Proteins to the Rough Endoplasmic Reticulum

Directing Proteins to the Rough Endoplasmic Reticulum

The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...

Initiation of Translation

Initiation of Translation

Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Ribosomal RNA Synthesis

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

Cotranslational Protein Translocation

Cotranslational Protein Translocation

Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...