接近红外激光的应用增加了视神经的电流值,与增加的Na+依赖运输相一致
Hin Heng Lo1, Tawan Munkongcharoen1, Rosa M Muijen1
1Neuroscience, Surgery and Trauma, Blizard Institute, QMUL, Whitechapel, London, E1 2AT, UK.
Pflugers Archiv : European journal of physiology
|February 29, 2024
概括
红外激光增加了视神经轴突中的电流值. 这种与轴突脱极化和流入相关的效应是由温度和-活动调节的.
科学领域:
- 神经科学是一个神经科学.
- 生物物理学的生物物理.
背景情况:
- 红外激光光线影响视神经轴突中的电流值的机制尚未完全理解.
- 以前的假设表明髓抗性下降,但这尚未得到证实.
研究的目的:
- 调查红外激光诱导的视神经轴突电流值增加背后的机制.
- 探索轴突膜潜力的作用,离子运输和髓抵抗.
主要方法:
- 应用红外激光光对隔离的老鼠视神经,在电刺激部位附近.
- 激光光的配对和单一应用,以评估值变化和恢复动力学.
- 利用Li+和布米坦化物来研究离子运输和折射性的作用.
- 测量神经输入电阻以评估髓抵抗贡献.
主要成果:
- 激光灯应用导致电流值迅速增加,重复应用后效果降低.
- 响应恢复表现出24秒的时间常数,而单次应用色的时间常数为40-50秒.
- +和布米坦化物部分阻断了值的增加,这表明轴突脱极化.
- 神经输入电阻测量排除了髓电阻的变化.
结论:
- 这些发现支持了轴突膜潜力取决于温度依赖的,电中性流入的模型.
- 激光诱导反应的淡化可能是由于细胞内的下降而导致Na+/K+-ATPase活性降低的结果.
- 红外激光光直接通过涉及离子运输的机制影响轴突刺激性.
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