相关实验视频

Updated: Jul 2, 2025

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

非甲酸途径需要一种微妙的中间体平衡,以最大限度地提高烯产量

Indu Raghavan¹, Rosheena Juman¹, Zhen Q Wang²

¹Department of Biological Sciences, University at Buffalo, the State University of New York, 653 Cooke Hall, Buffalo, New York, NY14260, USA.

Applied microbiology and biotechnology

|February 29, 2024

概括

具有稳定,基因组集成的甲基基酸盐 (MEP) 途径的工程大肠杆菌有效地产生烯. 微调途径中间体平衡流量,以提高基拉尼的生产,改善工业化工合成.

科学领域:

代谢工程是代谢工程.
合成生物学合成生物学
微生物生物技术微生物生物技术

背景情况:

烯是有价值的工业化学品,需求日益增长.
生物工程大肠杆菌是烯生产的关键策略.
甲基酸 (MEP) 途径在合成中提供了比酸 (MVA) 途径更高的效率.

研究的目的:

使用MEP途径在大肠杆菌中设计一个稳定的,无标记物烯生产系统.
为了优化MEP路径流量,以提高单基拉尼的生产.
为了研究MEP途径中间体和烯产量的关系.

主要方法:

整个MEP途径的基因组整合到大肠杆菌中.
使用各种促进剂调节通路基因转录.
量化MEP途径中间体和分析基拉尼奥尔度的量化.
主要成分分析 (PCA) 用于识别关键路径中间体.

主要成果:

与基于等离子体的系统相比,基因组集成的MEP途径显示出更高的菌株稳定性和兰醇生产.
使用中强度促进剂优化MEP通路基因 (dxs,idi,ispDF) 的表达产生了最高的geraniol.
高基拉尼生产者表现出高的异烯酸 (IPP) 和二甲基烯酸 (DMAPP) 水平.
根据PCA的数据,1-deoxy-D-xylulose 5-phosphate (DXP) 的含量是关键的,而MEP的含量对geraniol的含量不那么重要.

结论:

均衡的MEP途径对于最大限度地提高工程化大肠杆菌中的烯产量至关重要.
基因组集成的MEP路径为烯生产提供了稳定高效的底盘.
这项研究为通过代谢工程生产各种烯奠定了基础.

关键词:

一-脱氧-D-烯酸 5-酸盐反抑制可以抑制反.流量平衡的流量平衡.杰拉尼奥尔 (Geraniol) 是一种植物.甲基二酸盐路径的甲基二酸盐路径.

更多相关视频

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

Published on: May 16, 2014

相关概念视频

C4 Pathway and CAM

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...

The Citric Acid Cycle: Output

The Citric Acid Cycle: Output

The citric acid cycle is termed an amphibolic pathway as it operates both anabolically and catabolically. The cyclic reactions balance the flux of the substrates to provide an optimal concentration of NADH and ATP to the cell.
Regulation of Citric Acid Cycle
The citric acid cycle is regulated in several ways, including feedback inhibition, regulation of enzyme activities, and associated anaplerotic or cataplerotic pathways.
The primary substrate of the TCA cycle—acetyl CoA—is...

The Calvin Benson Cycle

The Calvin Benson Cycle

Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...

The Calvin Cycle

The Calvin Cycle

Overview of Fatty Acid Metabolism

Overview of Fatty Acid Metabolism

Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...

Olefin Metathesis Polymerization: Acyclic Diene Metathesis (ADMET)

Olefin Metathesis Polymerization: Acyclic Diene Metathesis (ADMET)

Acyclic diene metathesis polymerization or ADMET polymerization involves cross-metathesis of terminal dienes, such as 1,8-nonadiene, to give linear unsaturated polymer and ethylene. As ADMET is a reversible process, the formed ethylene gas must be removed from the reaction mixture to complete the polymerization process.
Similar to cross-metathesis, ADMET also involves the formation of metallacyclobutane intermediate by [2+2] cycloaddition of one of the double bonds of a terminal diene with...