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一种新的丸丰富基因Spem2对于小鼠的精子生成和受精是必需的

Chaojie Li¹, Chunling Shen¹, Wenfeng Xiong¹

¹State Key Laboratory of Medical Genomics, Research Center for Experimental Medicine, Rui-Jin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200025, China.

Cellular and molecular life sciences : CMLS

|February 29, 2024

概括

SPEM2是一种新型基因,对男性生育能力和精子生成至关重要. 它的缺失导致精子发育和功能受损,导致小鼠不孕.

科学领域:

生殖生物学生殖生物学
遗传学遗传学是一个
细胞生物学细胞生物学

背景情况:

精子生成,即创造成熟精子的过程,对男性生育能力至关重要.
控制精子生成的分子机制尚未完全理解.
识别涉及的关键基因对于了解男性不孕症至关重要.

研究的目的:

调查新型丸丰富基因SPEM家族成员2 (Spem2) 在精子生成和男性生育能力中的作用.
阐明Spem2在精子发育和功能中的分子功能.

主要方法:

产生和分析Spem2-缺乏 (Spem2-/-) 的小鼠.
评估精子形态,数量,运动性和体内/体外受精能力.
蛋白质相互作用的分析和处理在 epididymal 精子.

主要成果:

精子2主要表达在平分体的男性生殖细胞中,并在哺乳动物中保存.
精子2-零小鼠由于精子生成受损而表现出男性不育,包括精子个性化缺陷,细胞质流失和体形成.
缺少Spem2显著降低精子数量和运动性,并损害了体内和潜在的体外受精.
精子2与多种精子蛋白相互作用,并且对于几个精子蛋白的加工和成熟是必需的.

结论:

SPEM2是小鼠精子生成和雄性生育的重要调节剂,对包括人类在内的其他哺乳动物有潜在的影响.
了解SPEM2的功能为治疗男性不孕症和开发非荷尔蒙避孕药提供了洞察力.

关键词:

细胞质去除细胞质去除细胞质液滴是一个细胞质液滴.基因淘汰赛基因淘汰赛基因淘汰赛男人生殖的生殖系统精子发生是精子生成.

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Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...