化剂的表达和固定,用于从乳杆菌植物和葡萄球菌lugdunensis的皮革厂废水处理:一项比较研究
Amballa Chaitanyakumar1,2, Prathap Somu3, Ramachandran Srinivasan4,5
1Department of Biotechnology, University Institute of Engineering and Technology, Guru Nanak University, Ibrahimpatnam, 501510, Telangana, India. chaitu.05543@gmail.com.
Applied biochemistry and biotechnology
|February 29, 2024
概括
这项研究表明,有毒的酸有效地转化为有价值的酸,使用来自Lactobacillus plantarum和Staphylococcus lugdunensis的固定微生物酶. 这些固定酶提供了可重复使用和稳定的解决方案,用于处理皮厂的废水.
科学领域:
- 生物技术是生物技术.
- 环境科学 环境科学
- 酵素工程是什么意思 酵素工程
背景情况:
- 农业工业排放,特别是来自皮革厂的排放,是由于高度的皮革而造成的水和土壤污染的主要来源.
- 酸是制厂污水中的有毒成分,对环境构成重大风险.
研究的目的:
- 开发一种高效的方法,使用固定微生物酶将酸转化为酸.
- 评估固定化酶在处理皮革厂废水中的稳定性,可重复使用性和性能.
主要方法:
- 酶基因 (tanLpl和tanA) 被从乳酸菌植物菌和斯塔菲洛科克斯Lugdunensis中分离出来.
- 基因被克隆,在BL 21宿主中表达,酶被固定在酸珠中.
- 固定化珠用于处理不同温度的蔬菜制厂废水,通过TLC和NMR进行分析.
主要成果:
- 两种固定酶 (tanLpl和tanA) 都有效地将酸转化为酸,证实了素的减少.
- 固定化的酶表现出极好的可重复使用性,可长达15个周期,活动损失最小.
- 在金属离子存在的情况下,酶活性保持稳定,tanA表现出广泛的pH稳定性,而tanLpl表现出高的热稳定性.
结论:
- 固定化微生物酶为制厂废水的生物修复提供了一个有希望和可持续的解决方案.
- 固定式tanLpl和tanA之间的选择可以根据特定的工业废水组成和反应条件量身定制.
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