在城市污水中检测和量化腺病毒,多种病毒和乳头瘤病毒
Mohamed N F Shaheen1, Nehal Ahmed2, Kareem Rady Badr2
1Environmental Virology Laboratory, Department of Water Pollution Research, Environment and Climate Change Research Institute, National Research Center, 12622 Dokki, Cairo, Egypt E-mail: m_nrc2007@yahoo.com; mn.shaheen@nrc.sci.eg.
Journal of water and health
|February 29, 2024
概括
人类腺病毒 (HAdV),多重瘤病毒 (HPyV) 和乳头瘤病毒 (HPV) 经常在城市污水中检测到,冬季的发病率更高. HAdV和HPyV显示出作为污水污染指标的潜力.
科学领域:
- 环境微生物学环境微生物学
- 病毒学 病毒学
- 公共卫生 公共卫生
背景情况:
- 城市污水含有各种各样的病毒病原体.
- 环境监测提供了对社区级病毒循环的洞察力.
研究的目的:
- 确定城市污水中人类腺病毒 (HAdV),人类多瘤病毒 (HPyV) 和人类乳头瘤病毒 (HPV) 的发生率和季节性频率.
- 评估这些病毒是否适合作为污水污染指标.
- 探索环境监测对识别流传病毒的有用性.
主要方法:
- 聚合酶连锁反应 (PCR) 用于病毒检测.
- 定量PCR (qPCR) 用于病毒载量的量化.
- 基于PCR的HAdV和HPyV的基因型鉴定.
主要成果:
- 总共有63.3%的样本检测出HAdV,HPyV或HPV的阳性.
- 最常见的是HAdV (43.3%),其次是HPyV (35%) 和HPV (35%).
- 病毒度差异很大,检测率在冬季最高. 确定了HAdV类型40/41和HPyV类型JC/BK.
结论:
- HAdV和HPyV在城市污水中普遍存在,并适合作为便污染的指标.
- 环境监测对于监测社区传播的病毒是有效的.
- 这项研究强调了了解废水中的病毒存在对公共卫生的重要性.
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