功能性巨细胞与人类诱导的多能干细胞区分的协议
Suji Jeong1, Heesoon Chang2, Seok-Ho Hong3
1Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea.
STAR protocols
|February 29, 2024
概括
这项研究详细介绍了一种无料的协议,用于从人类诱导的多能干细胞 (hiPSCs) 产生功能性巨细胞. 这些hiPSC衍生的巨细胞对于疾病建模和药物发现应用非常有价值.
科学领域:
- 干细胞生物学 干细胞生物学
- 免疫学 免疫学 免疫学
- 细胞分化的细胞分化.
背景情况:
- 人类诱导的多能干细胞 (hiPSCs) 为产生专门细胞类型提供了可再生来源.
- 巨细胞在免疫,炎症和疾病发病过程中发挥着关键作用.
- 从hiPSCs生成巨细胞的现有方法可能是复杂的或低效的.
研究的目的:
- 建立一个强大且无料的协议,将hiPSC分化为功能性巨细胞.
- 为生成和表征hiPSC衍生的巨细胞提供详细的方法.
- 为了促进hiPSC衍生巨细胞在疾病建模和药物发现中的使用.
主要方法:
- 准备hiPSCs进行分化.
- 介质皮分化的无料诱导.
- 血液形成的承诺和随后的巨细胞分化.
- 不同化的巨细胞的扩张.
- 表型,偏振和细胞功能测试.
主要成果:
- 通过使用描述的协议,从hiPSC中成功生成功能性巨细胞.
- 巨细胞表型的表征,包括表面标记物.
- 证明巨细胞的极化能力 (例如,M1/M2).
- 在分化巨细胞中证实了细胞活性.
结论:
- 开发的协议提供了一种可靠的方法,用于从hiPSCs产生功能性巨细胞.
- 这些hiPSC衍生的巨细胞适用于疾病建模和药物发现的应用.
- 该协议允许创建用于高级研究研究的有机体.
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