使用RT-LAMP在 durum小麦原始叶子提取物中快速和具体检测小麦线条纹马赛克病毒
Monica Marra1,2, Paolo Mussano1, Eugenio Pinton1,3
1Institute for Sustainable Plant Protection, National Research Council, Turin, Italy.
PloS one
|February 29, 2024
概括
一种新的快速诊断试验小麦螺纹马赛克病毒 (WSSMV) 已经开发. 这种具有成本效益的方法可以在小麦中快速,灵敏地检测WSSMV,有助于了解其传播.
科学领域:
- 植物病毒学 植物病毒学
- 分子诊断学 分子诊断学
- 农业科学 农业科学
背景情况:
- 准确的病原体检测对于管理植物疾病至关重要.
- 小麦线串状马赛克病毒 (WSSMV),一种Bymovirus,显著降低小麦产量,并由Polymyxa graminis传播.
- 目前的WSSMV检测方法 (ELISA,PCR) 需要专门的实验室设置.
研究的目的:
- 为WSSMV开发一种快速,灵敏和具有成本效益的临床诊断试验.
- 优化反转录循环介导同热放大 (RT-LAMP) 协议用于在小麦中检测WSSMV.
主要方法:
- 开发和优化反转录循环介导的同热放大 (RT-LAMP) 协议.
- 测试RT-LAMP测定对WSSMV和相关病毒 (SBCMV,SBWMV) 的特异性.
- 对RT-LAMP测定与WSSMV特定的RT-PCR测定灵敏度的比较.
主要成果:
- 成功开发了一种特定的RT-LAMP协议,用于使用原始小麦叶提取物检测WSSMV.
- 该试验显示高特异性,没有SBCMV或SBWMV的交叉反应性.
- 该RT-LAMP试验的灵敏度与WSSMV特定的RT-PCR相提并论.
结论:
- 开发的RT-LAMP检测方法为WSSMV检测提供了一种快速,灵敏和特定的方法.
- 这种测试可以在现场条件下使用便携式设备进行,从而促进WSSMV传播的监测.
- 测试的可访问性可以帮助探索小麦中不太了解WSSMV的传播.
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