相关实验视频
Updated: Jul 2, 2025

10:24
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
83.5K
赋予未被赋予者:基于签名的rDNA元条码分类揭示了新的深海多样性
Inès Barrenechea Angeles1,2,3, Ngoc-Loi Nguyen4, Mattia Greco4,5
1Department of Earth Sciences, University of Geneva, Geneva, Switzerland.
PloS one
|February 29, 2024
概括
环境DNA元编码揭示了深海真核生物的多样性. 新的DNA签名有助于从类动物中分类未分配的数据,揭示了新的血统和它们在深海中的分布.
科学领域:
- 海洋生物学 海洋生物学
- 基因组学就是基因组学.
- 深海生态 深海生态
背景情况:
- 环境DNA (eDNA) 的元编码揭示了广泛的海洋真核生物多样性.
- 由于有限的参考数据库,特别是对于深海生物,大多数eDNA数据仍然未被分配.
- 深海生物群和微生物群的多样性在很大程度上尚未被探索.
研究的目的:
- 使用独特的DNA签名,从深海虫中分类未分配的元条码.
- 探索深海真核生物的隐藏多样性和分布.
- 通过metabarcoding提供环境监测的见解.
主要方法:
- 分析了来自克拉里昂-克利珀顿断裂区311个深海沉积物样本的eDNA元编码数据.
- 在18S rRNA基因的37F超变区中开发和应用DNA特征.
- 遗传学分析以确定新的血统并与现有数据集进行比较.
主要成果:
- 成功地将802个未被分配的元标码分类为27个族系学分类中的61个新型的氨基基线系.
- 大多数新型血统都广泛分布在深海.
- 在深海和浅水数据集中确定了五种血统,除了一个例子外,有明显的基因分离.
结论:
- 基于签名的分类提供了一种无分类方法来解释未分配的eDNA数据.
- 这种方法为深海物种提供了有价值的生态和分布见解.
- 虽然它没有解决参考数据库的缺口,但它增强了环境监测的深海生物多样性评估.
相关概念视频
Evolutionary Relationships through Genome Comparisons
5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.7K
Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
RNA-seq
10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K
Gene Evolution - Fast or Slow?
7.1K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
7.1K

