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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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热诺斯蒂克光探头 热诺斯蒂克光探头

Amit Sharma1, Peter Verwilst2, Mingle Li3

  • 1Amity School of Chemical Sciences, Amity University Punjab, Sector 82A, Mohali 140 306, India.

Chemical reviews
|February 29, 2024
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概括
此摘要是机器生成的。

光感应探针为药物输送提供时空控制,通过光信号释放药物. 本综述涵盖了最近的进展,特别是瘤微环境和其他疾病.

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科学领域:

  • 生物医学工程 生物医学工程
  • 分子成像学分子成像学
  • 药物输送系统 药物输送系统

背景情况:

  • 光感应探针集成成像和治疗功能,用于精确的药物输送.
  • 近年来,研究发表的关于theranostic光探针的研究出版物显著增加.
  • 这些探头通常由与掩盖药物连接的光体组成,在特定的刺激时释放它.

研究的目的:

  • 审查最近在光探测器的进步.
  • 专注于在瘤微环境中激活的探针.
  • 总结神经退行性疾病和抗菌应用的探针的发展.

主要方法:

  • 关于theranostic光探针的最新科学文献的审查.
  • 对探头设计的分析,包括小分子系统和纳米材料.
  • 由内在和外在刺激激活的突出探测器.

主要成果:

  • 光感应探头可以获得时空信息并控制药物输送.
  • 光信号表明药物释放,作为一个时空标记.
  • 光素促进治疗应用,如光动力学,光热和声动力学疗法.

结论:

  • 光探针的设计多样化,具有显著的临床影响潜力.
  • 专注于瘤微环境激活的探针是一个关键的研究领域.
  • 应用范围扩展到神经退行性疾病和抗菌治疗,显示出广泛的实用性.