脊髓基因表达的细胞因子在多个时间点在层次的脂多糖化物挑战之后的时间点
Mingyan Jing1, Shengnan Li2, Shusheng Zhao1
1Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, Canada.
American journal of veterinary research
|February 29, 2024
概括
在产母中注射的脂聚糖 (LPS) 显著增加了脏细胞因子mRNA和血清IL-6水平,在2小时内达到峰值. 这为对LPS的免疫反应提供了关键的见解.
科学领域:
- 禽类免疫学 禽类免疫学
- 炎症反应是一种炎症反应.
- 分子生物学分子生物学
背景情况:
- 脂聚糖 (LPS) 是格拉姆阴性细菌的关键成分,已知可以引起强烈的炎症反应.
- 了解家禽的这些反应对于动物健康和疾病管理至关重要.
研究的目的:
- 为了研究由产母注射脂多糖 (LPS) 引发的炎症级联.
- 描述LPS后细胞因子基因表达和血清细胞因子水平的时间动态.
主要方法:
- 30只40周大的产卵母被注射了LPS (8 mg/kg) 或无菌盐水.
- 在注射后的多个时间点 (0,1,2,3,4,6小时) 收集了血液和脏组织.
- 关键细胞因子 (IL-1β,IL-2,IL-6,IL-8,IL-10,IFN-γ,TNF-α) 和血清IL-6的脏mRNA水平被量化.
主要成果:
- 通过LPS注射显著提高了IL-1β,IL-2,IL-6,IL-8,IL-10,IFN-γ和TNF-α的脏mRNA水平 (P < .001).
- 在接受LPS治疗的母中,血清IL-6水平也显著升高 (P < .01).
- 细胞因子基因表达在2小时内达到峰值,IL-6显示出最明显的上调,其次是IL-1β和IL-8.
结论:
- 这项研究阐明了LPS挑战后中炎症媒介的时间和数量概况.
- 结果确定了最佳时间窗口和潜在的生物标志物,用于评估家禽中LPS诱导的炎症.
- 这些信息对于评估针对免疫系统的干预措施至关重要.
相关概念视频
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
01:58Cell Specific Gene Expression
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
01:05Insulin Secretory Vesicles
Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
01:22Diversity in Cell Signaling Responses
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity.
Graded and Abrupt Responses
Some signaling systems generate...
Graded and Abrupt Responses
Some signaling systems generate...
01:27Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are co-secreted in...
Insulin and C-peptide are co-secreted in...