相关实验视频

Updated: Jul 2, 2025

Strategies for Assessing Autistic-Like Behaviors in Mice

Strategies for Assessing Autistic-Like Behaviors in Mice

Published on: September 20, 2024

慢性Rapamycin通过饮用水的管理减轻了Krabbe疾病小鼠模型中的病态表型,通过自性激活

Ambra Del Grosso¹, Sara Carpi², Miriam De Sarlo²

¹Istituto Nanoscienze - CNR, Pisa, Piazza San Silvestro 12, Pisa 56127, Italy; Laboratorio NEST, Scuola Normale Superiore, Piazza S. Silvestro 12, 56127, Pisa, Italy.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

|February 29, 2024

概括

拉帕素 (RAPA) 治疗改善了运动功能,并减少了克拉贝病 (KD) 的小鼠模型中毒性精神素 (PSY) 的积累. 这表明RAPA可能是KD患者的潜在辅助疗法.

科学领域:

神经科学是一个神经科学.
生物化学生物化学
遗传学是一个遗传学.

背景情况:

克拉贝病 (KD) 是一种致命的神经退行性疾病,由银糖胺酶 (GALC) 缺乏引起,导致有毒精神病 (PSY) 积累.
通过p62聚合物证明的自功能障碍与KD病变发生有关.
之前的体外研究表明,拉帕 (RAPA) 降低了KD细胞中的p62聚合物.

研究的目的:

在Krabbe疾病 (KD) 的Twitcher (TWI) 鼠标模型中研究Rapamycin (RAPA) 的治疗潜力.
评估RAPA对TWI小鼠运动功能的影响,PSY水平,自标志物,星症和髓化.

主要方法:

在TWI小鼠中,从出生后的21-23天开始,RAPA (15 mg/L) 加入到饮用水中.
纵向监测包括运动性能测试 (握力,旋转杆).
生物化学分析评估了PSY积累,自标志物 (p62),星,并通过西部斑块和免疫组织化学髓化.

主要成果:

在特定时间点,RAPA治疗显著改善了TWI小鼠的运动功能.
在TWI小鼠的大脑,脊髓和坐骨神经中,RAPA降低了p62聚合物和星化.
在RAPA治疗后,在脊髓中观察到PSY积累的部分减少.

结论:

拉帕 (RAPA) 显示出作为Krabbe病 (KD) 辅助治疗的治疗潜力.
雷帕能够改善运动功能,减少神经炎症和减少有毒代谢物积累的能力需要进一步调查.
鉴于RAPA的临床可用性,其用于KD治疗的潜力可以在临床试验中迅速评估.

关键词:

全球细胞白血病变异性.Krabbe 疾病癌症癌症 P62聚合物的聚合物拉帕米辛是一种可怕的药物.这是一个Twitcher鼠标.自的诱导自的诱导精神病症是一种精神病.

更多相关视频

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases

Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases

Published on: September 6, 2024

相关概念视频

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...