相关实验视频

Updated: May 2, 2026

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

Published on: June 12, 2013

铁素功能化的多多巴胺薄膜通过适应性可湿性及时调解M1-to-M2巨细胞两极分化

Ying Shu¹, Kai Li², Jieping Li²

¹School of Materials and Chemistry, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai, China; Key Laboratory of Inorganic Coating Materials CAS, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, China.

Colloids and surfaces. B, Biointerfaces

|February 29, 2024

概括

植入物上的铁素修饰的多多巴胺膜通过清理活性氧物种 (ROS) 来促进M2巨细胞的两极分化. 这种表面修饰有助于平衡免疫反应和组织修复.

科学领域:

生物材料科学生物材料科学
免疫学免疫学免疫学
表面化学表面化学

背景情况:

控制巨细胞两极分化 (M1到M2) 对于骨科植入物成功至关重要,平衡免疫力和组织修复.
过度的活性氧物种 (ROS) 加剧了M1极化和植入部位的炎症.
开发响应炎症微环境的智能植入物表面是M1-M2过渡的关键.

研究的目的:

为了设计具有ROS响应和ROS清除能力的 (Ti) 植入表面.
为了研究铁基改性多多巴胺 (PDA-Fc) 薄膜对巨细胞两极分化的影响.
评估PDA-Fc修饰的Ti植入物在促进抗炎M2巨细胞表型方面的潜力.

主要方法:

用铁素修饰的多多巴胺 (PDA-Fc) 薄膜沉积在用等离子体喷涂的Ti (PST) 涂层上.
塑喷涂Ti (PST) 和PDA修改的PST (PST/PDA) 涂层作为对照.
评估了自由基清除,适应性可湿性 (对H2O2的反应),以及由脂多糖诱导的巨细胞极化 (M1到M2).

主要成果:

经过PDA修改的涂层 (PST/PDA和PST/PDA-Fc) 显示出自由基清除能力.
PST/PDA-Fc表现出适应性可湿性,在H2O2处理时变得更加水友.
与PST/PDA相比,PST/PDA-Fc显著增强了M1-M2巨细胞两极分化,通过增加CD206+细胞,细胞延长和阿基因酶1表达来表明这一点.

结论:

经过PDA-Fc修改的Ti涂层具有ROS清除和ROS响应的特性.
这些功能化的表面有效地促进了M2巨细胞的两极分化,从M1的表型转变.
这种方法为调节植入物表面的免疫反应提供了一个有希望的策略,以改善组织整合.

关键词:

铁烯是一种铁烯.巨细胞的两极分化聚多巴胺胺是一种多多巴胺.有反应性氧物种的反应性氧物种.湿透性湿透性湿透性

更多相关视频

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

Polarization and Characterization of M1 and M2 Human Monocyte-Derived Macrophages on Implant Surfaces

Polarization and Characterization of M1 and M2 Human Monocyte-Derived Macrophages on Implant Surfaces

Published on: December 6, 2024

相关概念视频

Mechanism of Filopodia Formation

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...