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便miRNAs的调节突出了地中海低炎症饮食干预措施的预防作用

Oscar Illescas¹, Giulio Ferrero², Antonino Belfiore³

¹Molecular Epigenomics Unit, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.

Clinical nutrition (Edinburgh, Scotland)

|February 29, 2024

概括

一种低炎症的地中海饮食改变了家族腺瘤多发症患者的肠道微RNA和基因表达,通过向炎症来预防癌症.

科学领域:

胃肠道学和瘤学
分子生物学分子生物学
营养科学营养科学

背景情况:

饮食干预措施正在探索用于癌症治疗.
在FAP (家族性腺多重症) 中的一项试点研究表明,低炎症的地中海饮食减少了炎症标志物.
这项研究调查了饮食对肠道微RNA (miRNA) 和基因表达的影响.

研究的目的:

为了确定低炎症地中海饮食是否调节便miRNA和肠道中的基因表达.
评估饮食对FAP患者miRNome和转录组的影响.

主要方法:

小RNA测序分析了在基线,3个月和6个月的便miRNA变化.
RNA测序评估了基线和6个月的直肠粘膜和腺瘤转录组变化.
在in silico方法中,确定了miRNA-基因相互作用,并分析了miRNA目标.

主要成果:

这种饮食调节了29种便miRNA,影响持续了干预后3个月.
与饮食坚持和炎症标志物 (calprotectin) 相相关的特定miRNAs.
70个基因在腺瘤和正常组织之间被差异地表达,在饮食后正常化;调节的miRNA和基因针对炎症途径.

结论:

由饮食调节的便miRNAs向与炎症相关的基因.
饮食引起的miRNA和具有瘤/瘤抑制功能的基因表达的变化表明地中海饮食的癌症预防作用.

关键词:

家庭性腺多重症 (FAP) 是一种家族性腺多重症.肠道组织的基因表达.地中海低炎症干预饮食小RNA测序小RNA测序是一个很好的方法.便中的微RNA概况

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