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RFEM:基于旋转森林和多特征融合的小鼠基本微RNA识别的框架
Shu-Hao Wang1, Yan Zhao2, Chun-Chun Wang3
1School of Information and Control Engineering, China University of Mining and Technology, Xuzhou, 221116, China; Artificial Intelligence Research Institute, China University of Mining and Technology, Xuzhou, 221116, China.
Computers in biology and medicine
|February 29, 2024
概括
一种新的计算方法,即基本微RNA识别 (RFEM) 的旋转森林,可以准确地预测小鼠的基本微RNA (miRNA). 该工具通过识别具有可靠性能的关键miRNA来增强miRNA研究.
科学领域:
- 生物信息学是一种生物信息学.
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 鉴定必要的微RNA (miRNA) 由于它们的数量不断增加而至关重要.
- 对于基本miRNA识别的现有计算方法是有限的.
研究的目的:
- 开发一种新的计算框架,即基本微RNA识别 (RFEM) 的旋转森林,用于预测小鼠中的miRNA基本性.
- 为了提高基本miRNA预测的准确性和可靠性.
主要方法:
- 通过融合来自miRNA-目标基因相互作用的现有和新型miRNA功能特征,构建了1,264个miRNA特征.
- 训练了一个旋转森林模型,使用182个样本和1264个特征来预测miRNA基本性得分.
- 引入了miRNA的功能特征,并利用了旋转森林的组合能力来加强分类.
主要成果:
- 在5倍交叉验证中,RFEM取得了显著的性能,在5倍交叉验证中,其AUC (AUPR) 为0.942 (0.944) 超过了之前的模型.
- 废除研究证实了新型miRNA功能特征的有效性.
- 案例研究表明,前10个预测的必需miRNA中的7个已经证实了小鼠的关键生物功能.
结论:
- RFEM是一种可靠和有效的工具,用于识别必要的miRNAs.
- 微RNA功能特征和旋转森林模型的整合显著提高了预测准确性.
- 在推进miRNA研究和理解生物功能方面,RFEM具有实际应用.
相关概念视频
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...